原发性干燥综合征患者血清白细胞介素-18水平测定及其意义

摘要

目的 通过检测原发性干燥综合征(PSS)患者血清白细胞介素-18 (IL-18)的水平,探讨其水平变化与PSS患者γ球蛋白的关系以及其在PSS发病中的作用.方法 选取20例符合PSS国际分类标准(2002年修订)的初诊PSS患者.采用双抗体夹心ELISA方法检测该组患者及l8例正常对照组血清中的IL-18水平.采用醋酸纤维薄膜法测定γ球蛋白水平.结果 PSS患者血清IL-18水平为(216.2 643 ±85.251) pg/ml,明显高于正常对照组水平(39.168 ±7.897) pg/ml.而且PSS患者中IL-18水平与γ球蛋白(23.205±2.5804)％存在明显正相关(r=0.538,P＜0.05).结论 PSS患者血清IL-18水平明显升高,且与γ球蛋白存在正相关,提示IL-18可能参与PPS的发病过程.%Objective This study explore the relationship between the alterative level of interleukin-18(IL-18) and γ globulin in serum and its role in predicting primary Siogren' s syndrome (PSS) by determining the level of serum IL-18 in patients with PSS. Methods The level of serum IL-18 were determined by enzyme linked immunologic sorbent assay (ELJSA) ; and the level of γ globulin were measured by cellulose acetate membrane method. Results The level of serum IL-18 in patients with PSS were (216. 2643 ± 85. 251) pg/ ml, much higher than that of normal control group (39. 168 ± 7. 897) pg/ml. And the level of IL-18 in patients with PSS is positively correlated with that of γ globulin. ( r = 0. 538, P < 0. 05 ). Conclusion The level of serum IL-18 in patients with PSS were much higher, and positively correlated with that of γ globulin, these results showed that IL-18 contributed to the pathogenesis of PSS.