农杆菌介导木薯遗传转化过程中的几个影响因素

摘要

Cassava agrobacterium-mediated transformation system has been optimized. The best infective conditions are as follows:OD 600 of bacteria liquid concentration is 1.0, additive amount of AS is 150μm, the time of infection is 45 min, co-culturing time is 3 d.%对农杆菌介导的木薯转化体系进行优化，结果表明，农杆菌侵染木薯的最适条件为：菌液浓度OD600=1.0， AS添加量150μm，侵染时间45 min，共培养3 d。