血清微小RNA-21在扩张型心肌病患者中的表达及其临床意义

摘要

Objective To investigate the expression and clinical significance of plasma miRNA - 21 in patients with dilated cardiomyopathy ( DCM ) . Methods 42 DCM patients admitted to cardiology department of our hospital from June 2011 to December 2011 were selected as case group and 20 healthy people were selected as control group. The biochemical parameters, including serum Cr, hs -CRP, BNP were detected in the two groups. PCR method was used to detect the expression of serum miRNA - 21 and 2-ΔΔC1 comparison method was used to calculate expression quantity. LVEDD and LVEF were detected by heartrncolor ultrasound, and SBP and DBP were measured at the same time in the two groups. Pearson linear correlation analysis and multiple linear correlation analysis were then performed to analyze the correlation of the variables. Results The biochemical parameters between the two groups showed statistically significant differences ( P < 0. 05 ) . Heart color ultrasound parameters and SBP showed statistically significant differences ( P <0. 05 ) . The expression quantity of miRNA -21 in the case group was ( 3. 8rn±2.1) times higher than the control group and the difference was statistically significant ( t = 6. 87, P <0. 05 ) . Pearson correlation analysis showed that the expression of miRNA - 21 was positively correlated with disease course, LVEDD and hs - CRP ( rrn= 0. 376, 0. 480 and 0. 622, P < 0. 05 ) . Multiple linear correlation analysis showed that miRNA - 21 expression was positively correlated with disease cowrse and hs - CRP ( P <0. 01 ) . Conclusion The expression of miRNA - 21 is elevated in DCM patients. The longer the disease course, the higher the hs - CRP level, and the higher the expression of miRNA -21. It is of good value for the diagnosis of DCM and can be a new indicator for efficacy assessment and prognosis and a potential therapeutic target.%目的 观察血清微小RNA(miRNA)-21在扩张型心肌病患者中的表达,探讨其临床意义.方法 选择2011年6-12月在我院心内科住院的扩张型心肌病患者42例为病例组,另选择同期我院体检健康者20例为对照组.检测两组受检者生化指标,包括血清肌酐(Cr)、超敏C反应蛋白(hs-CRP)、脑钠肽(BNP);采用实时荧光定量聚合酶链反应(PCR)法检测血清miRNA-21表达水平,采用2-△△Ct比较法计算其相对表达量;对两组受检者进行心脏彩超检查,测量左室舒张末内径(LVEDD)、左室射血分数(LVEF);同时测量收缩压(SBP)、舒张压(DBP).采用Pearson线性相关分析及多重线性相关分析对变量间的关系进行分析.结果 两组生化指标(血清Cr、hs-CRP、BNP)比较,差异均有统计学意义(P<0.05);心脏彩超检查指标(LVEDD、LVEF)比较,差异均有统计学意义(P<0.05);SBP比较,差异亦有统计学意义(P<0.05).病例组miRNA-21相对表达量是对照组的(3.8±2.7)倍,差异有统计学意义(t=6.87,P<0.05).Pearson线性相关分析显示,miRNA-21表达水平与病程、LVEDD和hs-CRP水平呈正相关(r值分别为0.376、0.480和0.622,P<0.05).多重线性相关分析显示,miRNA-21表达水平与病程和hs-CRP水平呈正相关(P<0.01).结论 扩张型心肌病患者血清miRNA-21表达上调,病程越长、hs-CRP水平越高,其表达水平越高,对扩张型心肌病具有一定的诊断价值,有可能成为评估疾病疗效及预后的新指标或潜在的治疗靶点.