Identification of a gene responsible for the 60-day delay in flowering time of Arabidopsis

摘要

Identification of genes related to flowering-time in Arabidopsis is very important and meaningful contribution to the flowering process control.One late flowering mutant plant,which exhibits 60-day delay in flowering,was screened from Arabidopsis library of T-DNA insertion.Southern blotting was used to confirm the single copy of exogenetic T-DNA in the genome of the mutant.The flanking sequence of T-DNA insert was obtained by TAILPCR and then analyzed by BLAST to confirm that the insertion site locates at the sixth exon of AT2G19520.1 (FVE gene).FVE is considered as a classical flowering time gene in Arabidopsis.It is a component of the autonomous pathway that encodes AtMSI4,which is a putative retinoblastoma-associated protein.The late-flowering mutant is named as fve-4,which is similar to fve-3 of Columbia and allelic withfve-1 andfve-2 ofLandsberg erecta.Thefve-4 mutant's delay of flowering was longer than that offve-3 mutant,whose T-DNA insertion is located at the first exon of FVE gene,suggesting that the sixth exon of FVE gene may play a more important role in the control of floral transition.