Fn14、p-JAK2和 p-STAT3在食管鳞癌中的表达及其相关性

摘要

Objective To investigate the role of Fn14 with JAK2/ STAT3 pathway in esophageal squamous cell carcinoma (ESCC), and the expression of JAK2/ STAT3 molecules were detected and the correlation of Fn14 with JAK/ STAT was discussed. Methods Fn14,p-JAK2 and p-STAT3 expressions in tumor tissues were assessed using immunohistochemical(IHC) methods in 118 patients with resected ESCC, and the correlation of Fn14 expression with JAK2/ STAT3 expression was analyzed. Results The expres-sion of Fn14 was mainly located on cell membrane and the expression of p-JAK2, p-STAT3 were mainly located in cytoplasm and cell nucleus. The positive expression rates of Fn14, p-JAK2 and p-STAT3 protein in ESCC were 51. 7%(61/ 118), 50. 9%(60/ 118) and 57. 6%(68/ 118), which were higher than 4. 2%(5/ 118) of corresponding adjacent non-cancerous tissue with statistical difference(P<0. 05). There were significant differences for Fn14, p-JAK2, p-STAT3 expressions in pathological grades and TNM stages groups(P<0. 05), while there were no significant differences for Fn14, p-JAK2, p-STAT3 in sex and age (P>0. 05). The correlation of Fn14 with p-JAK2, p-STAT3 were obvious in pathological grades and TNM stages in ESCC(P<0. 05). Conclusion The Fn14, p-JAK2, p-STAT3 proteins were highly expressed in ESCC, which suggest that Fn14, p-JAK2, p-STAT3 would be likely to be associated with the development of ESCC.%目的：检测信号分子 JAK2／ STAT3在食管鳞癌组织样本中的表达，并探讨成纤维细胞生长因子诱导14（Fn14）表达与 JAK2／ STAT3信号通路的相关性。方法采用免疫组织化学法检测118例食管癌组织及配对正常食管黏膜组织中 Fn14、p-JAK2和 p-STAT3蛋白的表达情况。分析 Fn14与 p-JAK2、p-STAT3蛋白表达的相关性及三者表达与食管鳞癌临床病理特征的关系。结果 Fn14阳性表达主要定位于细胞膜和胞浆，p-JAK2、p-STAT3阳性表达主要定位于细胞质和胞核。Fn14、p-JAK2、p-STAT3在食管鳞癌中的阳性表达率分别为51．7％（61／118）、50．9％（60／118）、57．6％（68／118），均高于正常食管黏膜鳞状上皮组织的4．2％（5／118），差异有统计学意义（P＜0．05）。 Fn14、p-JAK2及 p-STAT3蛋白表达与分化程度、TNM分期有关（P＜0．05），而与性别、年龄均无关（P＞0．05）。 Fn14与 p-JAK2、p-STAT3在食管鳞癌不同分化程度组织中及不同TNM 分期中均呈正相关（P＜0．05）。结论 Fn14、p-JAK2、p-STAT3在食管鳞癌中高表达，可能与食管鳞癌的发生、发展有关。