婴儿型双歧杆菌对肠癌大鼠化疗性肠黏膜炎的影响∗

摘要

目的：探讨婴儿型双歧杆菌对肠癌大鼠化疗所致的肠道黏膜炎的疗效。方法用皮下注射二甲基肼联合直肠黏膜种瘤造模方法建立肠癌大鼠模型41只，随机分为对照组（生理盐水）13只、化疗组（5-氟尿嘧啶＋奥沙利铂）14只、双歧杆菌组（5-氟尿嘧啶＋奥沙利铂＋婴儿型双歧杆菌灌胃）14只，记录3组肠癌大鼠的腹泻、体重、绒毛高度、隐窝深度及促炎性细胞因子 IL-6、IL-1β、TNF-α的水平。结果41只大鼠中12只成功致瘤，对照组3只，化疗组5只，双歧杆菌组4只。在肠癌模型大鼠中，治疗72 h 后，婴儿型双歧杆菌可以阻止由化疗引起的体重降低（P＜0．05）；减轻化疗后引起的腹泻次数（1．43±0．53 vs．2．43±0．79，P＜0．050）；减轻由化疗所致的小肠黏膜炎症反应，使绒毛长度升高[379．37±53．80）μm vs．（335．33±89．54）μm，P＜0．05]，隐窝深度变浅[（219．22±38．05）μm vs．（331．24±69．24）μm，P＜0．05]；降低由化疗引起的肠道促炎性细胞因子 IL-6、IL-1β水平的升高（P＜0．05），亦可使 TNF-α水平降低，但与化疗组比较，差异无统计学意义（P＞0．05）。而血浆中促炎性细胞因子水平的差异均无统计学意义（P＞0．05）。结论婴儿型双歧杆菌可有效减轻肠癌大鼠化疗介导的黏膜炎。%Objective To investigate the influence and curative effect of bifidobacterium infantis(B.infantis) on intestinal mucositis of cancer rats induced by chemotherapy. Methods A model of cancer rats by subcutaneous injection of dimethyl hydrazine with rectal mucosa of cancer cells was created, and experimental rats were randomly divided into three groups: control group(saline), chemotherapy group(5-FU+oxaliplatin), bifidobacterium infantis group(5-FU+oxaliplatin+B.infantis). The changes of diarrhea, body weight, villus height, crypt depth and levels of the proinflammatory factors IL-1β, IL-6 and TNF-α were recorded. Results In 41 mice, 12 mice consctracted tumor successfully, including 3 mice in control group, 5 mice in chemotherapy group and 4 mice in bifidobacterium infantis group. In the model of cancer rats, after 72 hours treatment,B.infantis administration prevented the loss in body weight(P<0. 05). B.infantis reduced the occurrence of diarrhea(1. 43±0. 53 vs. 2. 43±0. 79,P<0. 05). Compared to the chemotherapy treated rats, B.infantis could attenuate the injuries of the morphology of intestine.B.infantis-treated rats in a villus height[(379. 37± 53. 80)μm vs.(335. 33±89. 54)μm,P<0. 05] and a crypt depth[(219. 22±38. 05)μm vs.(331. 24±69. 24)μm,P<0. 05]. Intestinal IL-1β, IL-6 and TNF-α levels, which increased significantly in all rats receiving chemotherapy, were lowered by B.infantis administra-tion except TNF-α level. However, there were no significant differences in plasma IL-1β, IL-6 and TNF-α levels. Conclusion The Bifidobacterium infantis effectively reduce intestinal injury due to chemotherapy-induced intestinal mucositis in cancer rats.