山东地区汉族冠心病患者13号染色体基因与冠心病易感性相关联的研究

摘要

Objective:To explore the relationship between chromosome 13 and coronary artery disease(CAD)in patients of Han nationa lity in Shandong area.rnMethods:Our work included 2 groups, CAD group, n = 156 and Control group, n= 1000 healthy volunteers. A total of 14 microsatellite markers on chromosome 13 spaced at approximate 10 cm were screened in 2 separate DNA pools for 2 groups. CLUMP software was performed to calculate and compare the differences in allele frequency of each locus between 2 groups.rnResults: Statistic differences of allele frequencies were found at D13S263( 13ql4. Ll)and D13S156( 13q22. L)loci between CAD group and Control group,P<0.05.rnConclusion; Chromosome 13 loci 13ql4. 11 and 13q22. 1 may contain CAD related gene or CAD related regulating factor in Han nationality in Shandong area of China.%目的:探讨山东地区汉族人群13号染色体上与冠心病易感性相关联的遗传位点.方法:采集经冠状动脉造影确诊的冠心病患者血液标本156例、对照组1 000例,在13号染色体上选取间隔为10 cm遗传距离的14个微卫星遗传标记,利用DNA混合池技术分别对两组样本进行基因组扫描,采用CLUMP软件对比两组间各位点等位基因频率的差异,分析13号染色体上冠心病的易感基因位点.结果:在D13S263 (13q14.11)和D13S156( 13q22.1)位点,冠心病组与对照组的等位基因频率差异有统计学意义(P＜0.05).结论:山东地区汉族人群13号染色体13q14.11及13q22.1区域中存在与冠心病易感性的关联,冠心病致病基因或调控因子可能位于其附近.