抗心律失常肽10对柯萨奇B3病毒感染小鼠原代心肌细胞缝隙连接蛋白43的影响

摘要

Objective: To observe the effect of anti-arrhythmic peptide 10 (AAP10) on connexin 43 (Cx43) expression in mice primary myocardial cell infected with coxsackie virus B3 (CVB3) by establishing mice viral myocarditis model. n Methods: A total of 10 BALB/C mice at the age of 1-3 days were used for primary myocardial cell culture, the cells were infected by 100 times of TCID50 CVB3 and then, cultured with different doses of AAP10. The cells were divided into 4 groups:Cell control group, Virus control group, Low dose APP10 group and High dose APP 10 group. The protein expression of Cx43 was examined by Western blot analysis. n Results: The protein expression of Cx43 in Low dose APP10 group (71.46 ± 2.69)%and High dose APP 10 group (83.56 ± 3.74)%were obviously higher than that in Virus control group (44.92 ± 4.49)%, and with the higher APP 10 intervention, the protein expression of Cx43 increased accordingly, all P<0.01. n Conclusion: The anti-arrhythmic peptide 10 may protect myocardial cells, increase Cx43 expression and reduce myocardial injury from viral myocarditis in experimental mice.%目的：建立原代心肌细胞柯萨奇B3病毒（CVB3）感染性病毒性心肌炎的模型，使用抗心律失常肽10(AAP10)对该疾病模型进行干预，观察其对小鼠原代心肌细胞及缝隙连接蛋白43（Cx43）的影响。n　　方法：选取出生1～3天的BALB/C小鼠10只进行原代心肌细胞培养，分别用不同剂量的抗心律失常肽10的培养液进行干预100倍组织细胞培养半数感染量（TCID50）CVB3感染的心肌细胞，将原代小鼠心肌细胞分为细胞对照组、病毒对照组、低剂量干预组、高剂量干预组4组，每组8孔，通过蛋白免疫印迹法测定细胞缝隙连接蛋白43的表达水平。n　　结果：缝隙连接蛋白43相对表达水平低剂量干预组[（71.46±2.69）%]和高剂量干预组[（83.56±3.74）%]明显高于病毒对照组[（44.92±4.49）％]，随着干预剂量增加，其缝隙连接蛋白43相对表达水平进一步显著增加，差异均有统计学意义（P均<0.001）。n　　结论：抗心律失常肽10可保护心肌细胞，增加缝隙连接蛋白43的表达，减少病毒性心肌炎的心肌损害。