钙磷诱导大鼠血管平滑肌细胞钙化的机制研究

摘要

Objective: To explore the effect of oxidative stress injury on the mechanism of vascular smooth muscle cell (VSMC) calciifcation induced by calcium and phosphorus in experimental rats. Methods: The VSMC calcification was induced by incubating the cells with calcium chloride (CaCl2) andβ-sodium glycerophosphate (β-GP) for 8 days, and the cells were divided into 4 groups: ① Control group, ② Calcification group,③ Calciifcation+H2O2 group, ④ Calciifcation+catalase group. The calcium nodule formation and calcium deposition in VSMC were detected by Alizarin red staining and o-cresolphthalein complexone method, the reactive oxygen species (ROS) was detected by DCFH-DA probe staining and the protein expression of Runx2 was examined by Western blot analysis. Results: Compared with Control group, Calciifcation group showed the higher ROS production, more calcium nodule and calcium deposition, higher Runx2 protein expression;while compared with Calciifcation group, the above indexes were even higher in Calciifcation+H2O2 group, P<0.05. The ROS production, calcium nodule, calcium deposition and Runx2 protein expression were lower in Calciifcation+catalase group than those in Calciifcation group and Calciifcation+H2O2 group, but still higher than that in Control group. The protein expression of Runx2 was similar between Calciifcation+catalase group and Control group, P>0.05. Conclusion: CaCl2 andβ-GP treatment may induce VSMC calciifcation via activating ROS-Runx2 signal pathway in experimental rats.%目的：探讨氧化应激损伤在钙磷诱导的血管平滑肌细胞（VSMCs）钙化过程中的作用机制。　　方法：采用氯化钙（CaCl2）联合β-甘油磷酸钠（β-GP）建立大鼠VSMCs钙化模型。实验分为4组，对照组、钙化组、钙化+过氧化氢（H2O2）组和钙化+过氧化氢酶组。8天后分别通过茜素红S染色法和邻甲酚肽络合酮比色法检测各组细胞钙结节形成及钙含量；活性氧检测试剂盒（DCFH-DA探针）检测各组细胞活性氧阳性细胞数；蛋白免疫印迹法检测各组细胞中成骨转录因子Runx2的蛋白表达量。　　结果：钙化组活性氧的产生、钙结节、钙含量及Runx2蛋白表达量均显著高于对照组，钙化+过氧化氢组与钙化组相比，各项指标进一步升高，差异有统计学意义（P<0.05）。钙化+过氧化氢酶组活性氧产生量、钙结节、钙含量及Runx2蛋白表达量均低于钙化组和钙化+过氧化氢组，仍高于对照组，差异有统计学意义（P<0.05），但其中Runx2蛋白表达量与对照组相比则差异无统计学意义（P>0.05）。　　结论：氯化钙联合β-GP通过激活ROS-Runx2信号通路诱导大鼠VSMCs钙化形成。