怀山药类原球茎的诱导形成与植株再生

摘要

To improve the speed of in vitro propagation of Dioscorea opposita and shorten the breeding cycle, we used nodal segments with axillary buds of D. opposita cv. 'Tiegun' as the starting material. The optimal conditions for protocorm-like body (PLB) induction, proliferation, differentiation and plantlet regeneration were analyzed. The optimal culture medium for PLB induction was MS+1.0 mg·L-1 TDZ+30 g·L-1 sucrose, the optimal culture medium for PLB proliferation was MS+9 mg·L-1 6-BA+30 g·L-1 sucrose, and the optimal culture medium for PLB differentiation was MS+2 mg·L-1 KT+0.02 mg·L-1 NAA+30 g·L-1 sucrose. In culture medium containing 1/4MS+0.05 mg·L-1 NAA+1.0 mg·L-1 PP333+15 g·L-1 sucrose, the rooting rate of regenerated plantlets from PLBs was 80％, and the survival rate of regenerated plantlets was up to 85％ after being sown in soil. The induction of protocorm like bodies and the establishment of plant regeneration system provide a new way for rapid propagation of D. opposita seedlings.%为提高山药离体繁殖的速度,缩短繁殖周期,以铁棍山药(Dioscorea opposita cv. 'Tiegun')带腋芽茎段为材料,对类原球茎的诱导、增殖、分化与植株再生进行了研究.结果表明,铁棍山药类原球茎诱导的最适培养基为MS+1.0 mg·L-1 TDZ+30 g·L-1蔗糖,增殖的最适培养基为MS+9 mg·L-1 6-BA+30 g·L-1蔗糖,分化的最适培养基为MS+2 mg·L-1 KT+0.02 mg·L-1 NAA+30g·L-1蔗糖,最适生根培养基为1/4MS+0.05 mg·L-1 NAA+1.0 mg·L-1 PP333+15 g·L-1蔗糖,生根率达80％,移栽成活率可达85％.类原球茎的诱导形成及植株再生体系的建立为怀山药种苗的快速繁殖提供了一条新途径.