大白菜-结球甘蓝单体异附加系试管苗生根能力的研究

摘要

With the plantlets of Chinese cabbage [Brassica compestris L.ssp.pekinensis ( Lour )Olsson]-cabbage ( Brassica oleracea L.var.capitata L.) monosomic alien addition lines as materials,comparison of rooting rate and transplanting survival rate among different monosomic alien addition lines and effect of NAA content in 1/2 MS medium on the root induction were conducted in this paper.The result showed that monosomic alien addition lines AA+C1, AA+C2, AA+C3, AA+C4, AA+C5, and AA+C6 had higher rooting and transplanting survival rate, whereas the rate were much lower for the AA+C7, AA+C8 and AA+C9, adding 1.5 mg · L-1 NAA in 1/2 MS could effectively induce the root initiation and raise the transplanting survival frequency of monosomic alien addition lines AA+C7,AA+C8 and AA+C9.%以大白菜-结球甘蓝系列单体异附加系试管苗为试材,比较了不同单体异附加系试管苗的生根率和移栽成活率,研究了不同浓度NAA对单体异附加系试管苗生根和根尖细胞分裂的影响.结果表明:AA+C、AA+C、AA+C、AA+C、AA+C和AA+C的试管苗生根较好,移栽成活率高,AA+C、AA+C和AA+C的试管苗不易生根,移栽成活率低;1/2MS培养基附加1.5 mg·mLNAA能有效诱导试管苗生根,提高试管苗生根质量和移栽成活率.