不同结球甘蓝品种硫代葡萄糖苷组分及含量分析

摘要

Taking 11 cabbage(Brassica oleracea L. var.capitata L.)varieties as experimental material,their glucosinolate profiles and contents in leaves were evaluated by HPLC method. The results indicated that 9 types of glucosinolate were existed in cabbage leaves,including 5 aliphatic glucosinolates (Glucoiberin,Sinigrin,Progoitrin,Glucoraphanin and Gluconapin),and 4 indolic glucosinolates(4-Hydroxy glucobrassicin,Glucobrassicin,4-Methoxy glucobrassicin and Neoglucobrassicin). Glucoiberin,Sinigrin and Glucobrassicin were the main glucosinolate profiles in cabbage leaves. The 3 glucosinolates ranged from 63.60%-91.06% of the total glucosinolate contents. The contets of total glucosinolate,total aliphatic glucosinolate and total indolic glucosinolate ranged 3.59-19.70 μmol·g-1(DW),2.74-15.15μmol·g-1(DW),and 0.81-7.19μmol·g-1 (DW),respectively.%采用HPLC法，对11个结球甘蓝叶片硫代葡萄糖苷（硫苷）组分及含量进行测定。结果表明：结球甘蓝叶片中共含有9种硫苷，分别为3-甲基硫氧烯丙基硫苷（IBE）、2-羟基-3-丁烯基硫苷（PRO）、2-丙烯基硫苷（SIN）、4-甲基硫氧丁基硫苷（RAA）和3-丁烯基硫苷（NAP）等5种脂肪族硫苷，4-羟基吲哚基-3-甲基硫苷（4OH）、3-甲基吲哚基硫苷（GBC）、4-甲氧基吲哚基-3-甲基硫苷（4ME）和1-甲氧基吲哚基-3-甲基硫苷（NEO）等4种吲哚族硫苷；IBE、SIN和GBC是结球甘蓝叶片中硫苷的主要组分，三者总量占总硫苷含量的63.60%～91.06%。结球甘蓝叶片总硫苷含量在3.59～19.70μmol·g-1（DW）之间，总脂肪族硫苷含量在2.74～15.15μmol·g-1（DW）之间，总吲哚族硫苷含量在0.81～7.19μmol·g-1（DW）之间。