CD44v6、ADA、IFN-γ联合检测在胸腹水鉴别中的临床应用

摘要

目的：探讨可溶性CD44v6(sCD44v6)、腺苷脱氨酶(ADA)、干扰素-γ(IFN-γ)联合检测对良、恶性胸腹水的鉴别诊断价值。方法取结核性胸腹水28例(结核组)、恶性胸腹水32例(恶性组)、炎性胸腹水20例(炎性组)，分别用酶联免疫吸附试验(ELISA)及化学法检测胸、腹水sCD44v6、ADA、IFN-γ水平。结果恶性组sCD44v6水平明显高于结核组及炎性组；结核组ADA、IFN-γ水平明显高于恶性组及炎性组，差异有统计学意义(P<0.05)。sCD44v6诊断恶性胸腹水的阳性率为87.5%；ADA诊断结核性胸腹水的阳性率为89.2%；IFN-γ在诊断结核性胸腹水时阳性率为96.4%。结论sCD44v6、ADA、IFN-γ联合测定对于良恶性胸腹水的鉴别诊断有重要价值。%Objective To investigate the value of combined detection of soluble CD44v6(sCD44v6),adenosine deaminase(ADA) and interferon-γ(IFN-γ) in differentiating malignant from benign pleuroperitoneal effusions.Methods Chose 28 cases of tuberculous pleuroperitoneal effusions(tuberculous group), 32 cases of malignant pleuroperitoneal effusions(malignant group), 20 cases of inflammatory pleuroperitoneal effusions(inflammatory group), sCD44v6, ADA, IFN-γlevel were detected respectively by enzyme-linked immunosorbent assay(ELISA) and chemical method.Results sCD44v6 level in malignant pleuroperitoneal effusions group was significantly higher than that in tuberculous and inflammatory pleuroperitoneal effusions group; the levels of ADA and IFN-γin tuberculous pleuroperitoneal effusions group was significantly higher in malignant and inflammatory pleuroperitoneal effusions group, the difference was statistically significant(P<0.05). The positive rate of sCD44v6 diagnosis of malignant pleural effusion was 87.5%; the positive rate of ADA in the diagnosis of tuberculous pleural effusion was 89.2%; IFN-gamma in the diagnosis of tuberculous pleuroperitoneal effusion, the positive rate was 96.4%.Conclusion CD44v6, ADA, IFN-γcombined detection has important value in differentiating malignant from benign pleuroperitoneal effusions.