OBJECTIVE: To develop a method of thin- layer chromatographic scanning to determine the content of puerarin in Jiangtang pill METHODS: Silica GF254 thin- layer was used, and the mobile phase consisted of chloroform- methanol- water(7∶ 2 5∶ 0 25) with the detection wave- length of 254nm and the control wave- length of 370nm RESULTS: The linear range of puerarin was 0 4~ 2 0μ g The mean recovery was 99 40% (n=5) with RSD of 1 37% CONCLUSION: This method is accurate, reliable and can be used for the determination of puerarin in Jiangtang pill%目的:建立薄层扫描法测定降糖丸中葛根素含量的方法。方法:固定相为硅胶 GF254,流动相为氯仿-甲醇-水( 7∶ 2 5∶ 0 25),检测波长为 254nm,参比波长为 370nm。结果:葛根素在 0 4μ g~ 2 0μ g范围内呈良好的线性关系,平均回收率为 99 4%( n=5), RSD为 1 37%。结论:本方法准确、可靠,可用于测定降糖丸中葛根素的含量。
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