茵陈素对肺癌细胞增殖和细胞周期的影响

摘要

目的：探讨茵陈素对肺癌细胞增殖和细胞周期的影响。方法：应用光镜、四氮甲唑蓝（ MTT）方法和流式细胞术分析茵陈素对肺癌细胞形态学、生长和细胞周期的变化。结果：茵陈素能抑制肺癌细胞的增殖，呈剂量依赖性，以 160μ g/ml抑制作用最明显，抑制率达 52 4％。 80μ g/ml时， S期和 G2/M期细胞比例开始下降，细胞被阻滞于 G0/G1期，不能进入 S期及 G2/M期，增殖指数明显下降。结论：茵陈素在体外对肺癌细胞具有抑制作用，通过抑制 DNA合成，将细胞阻滞于 G0/G1期来抑制细胞增殖。%AIM： To investigate the effects of 6， 7－ dimethoxycoumarins on the proliferation and the cell cycle of human lung carcinoma cell（ PA） in vitro METHODS： The effects of 6， 7－ dimethoxycoumarins on the cytomorphology， growth and cell cycle of PA cells were evaluated by MTT assay and flow cytometry in vitro RESULTS： 6， 7－ dimethoxycoumarins could inhibit the proliferation of PA cells in a dose－ dependent manner The inhibition action of 6， 7－ dimethoxycoumarins plateaued at 160μ g/ml(52 4％ inhibition) The agent in dose of 80μ g/ml led to a decline in the proportion of cells to those in G2/M phase， made the cells remain in G0/G1 phase and reduced the proliferation index(PI) of PA cells CONCLUSION： In vitro， 6， 7－ dimethoxycoumarins can inhibit the proliferation of PA cells， which leads to arrest of the cells in G0/G1 phase by way of inhibiting DNA synthesis