目的:研究苦菊乙酸乙酯提取物(CEE)的体外抗氧化活性.方法:苦菊经95%乙醇加热回流、石油醚脱脂和乙酸乙酯萃取,挥干乙酸乙酯后得到CEE.通过二苯代苦味酰自由基(DPPH·)清除能力、还原能力和2,2'-偶氮-双-(2-脒基丙烷)氯化二氢(AAPH)诱导的红细胞溶血模型,检测CEE的抗氧化活性.结果:在DPPH·清除能力试验中,CEE和阳性对照药的清除率为50%时所需待测液的浓度(EC(50))分别为(59.76±6.11) mg·L-1,和(3.89±0.23)mg·L-1,两者相差1.54个数量级.CEE和阳性时照药(抗坏血酸)皆能使还原能力测试体系的吸光度增加,并呈浓度-效应关系(P<0.01),但CEE的还原能力不如抗坏血酸(P<0.01).在AAPH诱导的红细胞溶血模型中,CEE能有效降低兔血红细胞的溶血率(P<0.01),且高剂量(20mg·L-1) CEE可使溶血率恢复到空白对照的水平.结论:CEE能有效清除自由基,具有一定的还原能力,并对AAPH诱导的兔红细胞溶血具有很好的保护作用.提示CEE具有一定的抗氧化活性,可对其抗氧化活性及其有效成分作进一步的研究.%OBJECTIVE: To study the antioxidant activity of ethyl acetate extract of Cichorium endivia( CEE ). METHODS: C.endivia was refluxed with 95％ ethanol, and then was extracted with petroleum ether and ethyl acetate. After evaporation of ethyl acetate, the residue of CEE was obtained. The antioxidant activity of CEE was measured by scavenging capacity and reducing capacity of 2, 2-diphenyl-1-picrylhydrazyl (DPPH·) and the model of rabbit erythrocyte hemolysis induced by 2,2' -azobis(2-amidinopropane) dihydrochloride (AAPH). RESULTS: In DPPH· scavenging capacity assay, the EC50s of CEE and trolox were (59.76 ± 6.11)mg·L-1 and (3.89±0.23) mg· L-1, respectively. They compared with each other had 1.54 orders of magnitude. CEE and ascobic acid both improved the absorbance in reducing capacity teat system, and showed the concentration-effect relationship (P＜0.01). But the reducing capacity of CEE was weaker than that of ascorbic acid(P＜0.01). In the model of rabbit erythrocyte hemolysis induced by AAPH, CEE decreased the hemolysis rate of rabbit erythrocyte(P＜0.01 ), and the high concentration of CEE (20 mg· L-1) could recover the hemolysis rate to the level of blank control group. CONCLUSION: The CEE shows the capacity of scavenging radical, a certain reducing capacity, and protection effect on the hemolysis of rabbit erythrocyte induced by AAPH.CEE exhibits a certain antioxidant capacity in vitro, which remains to be further studied on the other antioxidant activities and its related effective constituents.
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