灯盏乙素苷元对帕金森病模型大鼠多巴胺能神经元的保护作用研究

摘要

OBJECTIVE: To explore the protective effect of scutellarein on dopaminerigic neurons of parkinson' s disease(PD) model rats. METHODS: PD rat models were induced by stereotaxic injection of LPS. Model rats were divided into 7 groups, i.e. blank control group (constant volume of normal saline), sham operation group (constant volume of normal saline), model group (constant volume of normal saline), Madopar group (0.034 mg/kg) and scutellarein high-dose, medium-dose and low-dose groups (800, 400, 200 mg/kg). They were given medicine intragastrically once a day for consecutive 3 weeks. Apomorphine (apo) was injected subcutaneously on the rat back to observe praxiology of it. The levels of MDA, SOD and GSH were determined. RESULTS: Compared with model group, rotational speed of rats were decreased significantly in scutellarein high-dose, medium-dose and low-dose groups(P<0.05); GSH and SOD levels were increased significantly, while MDA level was decreased significantly(P< 0.01 or P<0.05). CONCLUSION: Scutellarein can improve rotation behavior of PD model rats and the ability of anti-oxidation of dopaminergic neurons and protect the central nervous system of PD rats.%目的:研究灯盏乙素苷元对帕金森病(PD)模型大鼠多巴胺能神经元的保护作用.方法:通过给予大鼠脑立体定位注射脂多糖(LPS)复制PD模型.实验分为空白对照(等容生理盐水)、假手术(等容生理盐水)、模型(等容生理盐水)、美多芭(0.034mg/kg)与灯盏乙素苷元高、中、低剂量(800、400、200mg/kg)组.灌胃给药,每天1次,连续3周.背部皮下注射阿朴吗啡诱导进行行为学观察；测定丙二醛(MDA)、超氧化物歧化酶(SOD)、谷胱甘肽(GSH)水平.结果:与模型组比较,灯盏乙素苷元高、中、低剂量组大鼠旋转速度显著减慢(P＜0.05)；SOD活性显著增强,GSH含量显著增加,MDA含量显著减少(P＜0.01或P＜0.05).结论:灯盏乙素苷元能改善PD模型大鼠的旋转行为,同时可以提高多巴胺能神经元的抗氧化能力,从而对PD大鼠的中枢神经系统发挥保护作用.