改良RP-HPLC法同时测定牡丹皮药材中芍药苷和丹皮酚的含量

摘要

目的:通过改良同时测定牡丹皮药材中芍药苷和丹皮酚含量的方法,为控制牡丹皮药材的质量提供更有效的手段.方法:色谱柱为Agilent ZORBAX Eclipse XDBC18(250mm×4.6 mm,5 μm),流动相为甲醇-水(梯度洗脱),流速为1.0 ml/min,双波长检测(芍药苷为230nm,丹皮酚为274 nm).结果:芍药苷、丹皮酚的进样量分别在0.060 33～1.206 6、0.200 4～4.008 0 μg范围内与各自峰面积积分值呈良好线性关系(r均为0.999 9)；精密度、重复性、稳定性试验的RSD均＜2％；平均加样回收率分别为99.49％、98.66％,RSD分别为2.0％、1.8％(n均为6).结论:改良方法去除了磷酸对色谱柱的腐蚀,方法简便、快速,结果准确,可为牡丹皮及其相关制剂中芍药苷和丹皮酚的定量分析提供参考.%OBJECTIVE: To provide more effective means for the quality control of Paeonia suffruticosa by improving the method for simultaneous determination of paeoniflorin and paeonol in P. suffruticosa. METHODS: Agilent ZORBAX Eclipse XDB C18 (250 mm×4.6 mm,5 μm) column was used with mobile phase consisted of methanol-water (gradient elution) at the flow rate of 1.0 ml/min. The detection wavelength was 230 nm for paeoniflorin and 274 nm for paeonol. RESULTS: The linear range of paeoniflorin was 0.060 33-1.206 6 μg and that of paeonol was 0.200 4-4.008 0 μg(r=0.999 9). RSDs of precision test,reproducibility test and stability test were all lower than 2%. The average recoveries were 99.49% and 98.66% with RSDs of 2.0% and 1.8% respectively (n=6). CONCLUSION: The modified method removes phosphate corrosion for chromatographic column. The method is simple, rapid and accurate, andean be used for the qualitative analysis of paeoniflorin and paeonol in P. suffruticosa and its preparations.