RP-HPLC法同时测定荷叶颗粒中3种有效成分的含量

摘要

目的：建立同时测定荷叶颗粒中芦丁、槲皮素、荷叶碱含量的方法。方法：采用反相高效液相色谱法。色谱柱为Hypersil ODS2，流动相为乙腈-水（含0.3％磷酸和0.4％三乙胺）（梯度洗脱），流速为1.0 ml/min，检测波长为256 nm，柱温为25℃，进样量为10μl。结果：芦丁、槲皮素、荷叶碱的检测进样量线性范围分别为0.012～0.240、0.0104～0.208、0.015～0.300μg（r均为0.9999）；精密度、稳定性、重复性试验的RSD＜2.0％；加样回收率分别为98.5％～101.3％、99.1％～101.6％、98.9％～101.7％，RSD分别为1.1％、1.0％、1.2％（n＝6）。结论：该方法操作简便、结果准确可靠，可为荷叶颗粒的质量控制提供参考。%OBJECTIVE:To establish a method for simultaneous determination of rutin,quercetin and nuciferine in Heye gran-ule. METHODS:HPLC was performed on the column of Hypersil ODS2 with mobile phase of acetonitrile-water(containing 0.3%phosphoric acid and 0.4% triethylamine)(gradient elution)at a flow rate of 1.0 ml/min,the detection wavelength was 256 nm,the column temperature was 25 ℃,and the injection volume was 10 μl. RESULTS:The linear range was 0.012-0.240 μg for rutin(r=0.999 9),0.010 4-0.208 μg for quercetin(r=0.999 9)and 0.015-0.300 μg for nuciferine(r=0.999 9);RSDs of precision,stability and reproducibility tests were lower than 2.0%;recoveries were 98.5%-101.3%(RSD=1.1%,n=6),99.1%-101.6%(RSD=1.0%, n=6)and 98.9%-101.7%(RSD=1.2%,n=6). CONCLUSIONS:The method is simple,accurate and reliable,and can provide ref-erence for quality control of Heye granule.