杜仲壮骨丸对维甲酸致小鼠骨质疏松的改善作用

摘要

OBJECTIVE:To investigate improvement effect of Duzhong zhuanggu(DZ)pills on retinoic acid-induced osteopo-rosis in mice. METHODS:72 KM mice were randomized into blank control group(normal saline),model group(normal saline), positive control group (Xianling gubao capsule,0.50 g/kg),DZ pills high-dose,medium-dose and low-dose groups (1.90,0.95,0.48 g/kg,DZ-H,DZ-M,DZ-L groups),with 12 mice in each group. Except for blank control group,other groups were given ret-inoic acid intragastrically to induce osteoporosis model,and given relevant medicine intragastrically once a day,for 14 days. After last administration,body weight of mice was determined;the blood sample was collected from eyeball to determine the contents of serum calcium and phosphor;liver,spleen and thymus gland were collected to determine organ index;left femur was collected to determine bone wet weight,bone dry weight,length and diameter;right femur was collected to determine the area percentage, width and number of bone trabecula. RESULTS:Compared with blank control group,the body weight of mice in model group were decreased;the contents of serum calcium and phosphorus were increased;liver indexes and spleen indexes were increased, while thymus indexes were decreased;bone wet weight,bone dry weight,length and diameter were decreased;area percentage, width and number of trabecular were decreased,with statistical significance (P<0.05 or P<0.01). Compared with model group, the contents of serum calcium were decreased in treatment groups,and the contents of serum phosphorus were decreased in positive control group,DZ-M and DZ-L groups. Thymus indexes were increased in positive control group,DZ-M and DZ-L groups,while liver indexes were decreased in positive control group;femur wet weight of positive control group as well as femur dry and wet weight,diameters of DZ-M were all increased. The percentage,width and number of bone trabecula were increased in positive con-trol group,DZ-H and DZ-M group,and the number of bone trabecula was increased in DZ-L group,with statistical significance (P<0.05 or P<0.01). CONCLUSIONS:DZ pills have improvement effect on osteoporosis induced by retinoic acid in mice.%目的:考察杜仲壮骨丸(DZ)对小鼠骨质疏松的改善作用.方法:将72只KM小鼠随机分为空白对照组(生理盐水)、模型组(生理盐水)、阳性对照组(仙灵骨葆胶囊,0.50 g/kg)和DZ高、中、低剂量组(1.90、0.95、0.48 g/kg,记为DZ-H、DZ-M、DZ-L组),每组12只.除空白对照组外,其余各组小鼠均ig维甲酸复制骨质疏松模型,并同时ig相应药物,每天1次,连续14 d.末次给药后,测定小鼠体质量;摘眼球取血测定血清钙、血清磷含量;取肝、脾、胸腺测定脏器指数;取左侧股骨测定骨湿质量、干质量、长度和直径;取右侧股骨测定骨小梁面积百分比、骨小梁宽度及数目.结果:与空白对照组比较,模型组小鼠体质量降低;血清钙、血清磷含量增加;肝指数、脾指数升高,胸腺指数降低;股骨湿、干质量及直径减少;骨小梁面积百分比降低,骨小梁宽度及数目减少,差异均有统计学意义(P<0.05或P<0.01).与模型组比较,各给药组小鼠血清钙含量减少,阳性对照组和DZ-M、DZ-L组小鼠血清磷含量减少;阳性对照组和DZ-M、DZ-L组小鼠胸腺指数升高,阳性对照组小鼠肝脏指数降低;阳性对照组小鼠的股骨湿质量及DZ-M组小鼠股骨干、湿质量和直径均增加;阳性对照组和DZ-H、DZ-M组小鼠股骨骨小梁百分比升高,骨小梁宽度及骨小梁数目均增加,且DZ-L组小鼠骨小梁数目增加,以上差异均有统计学意义(P<0.05或P<0.01).结论:DZ对维甲酸致小鼠骨质疏松具有一定的改善作用.