目的:探讨酶放大免疫测定技术(EMIT)检测环孢素血药浓度的动态飘逸校正系数.方法:针对EMIT法检测环孢素药物浓度的动态飘逸现象,以质量浓度为100 ng/mL的环孢素定标液为待测样品,选择其第58、101次检测结果来进行动态飘逸相关系数的推拟;分别以上述系数对第1、20、45、77、106、115次检测结果进行校正,考察校正质量浓度与真实质量浓度的差异.结果:推拟得动态飘逸系数k=(1+0.001235×n0.4195)n,动态飘逸校正系数k′=1/[(1+0.001235×n0.4195)n].各次检测结果经校正后,其校正质量浓度与真实质量浓度的相对误差在±4%之内.结论:推拟所得的动态飘逸校正系数可用于环孢素质控样品浓度的动态校正,有助于环孢素血药浓度的准确检测.%OBJECTIVE:To explore the dynamic upwarp state correction coefficient of cyclosporine blood concentration determined by EMIT. METHODS:The dynamic upwarp state of cyclosporine concentration was determined by EMIT. The cyclosporine calibration reagents with concentration of 100 ng/mL was used as the sample to be measured,the 58th and 101st test results were selected to simulate the dynamic upwarp state related coefficients,and the 1st,20th,45th,77th,106th,115th test results were corrected by above coefficients. The difference of correction quality concentration with real quality concentration was investigated. RESULTS:The dynamic upwarp state coefficient [k=(1+0.001 235×n0.419 5)n] and dynamic upwarp state correction coefficient {k′=1/[(1+0.001 235×n0.419 5)n} were obtained. After determination results were corrected,relative error of corrected quality concentration with real quality concentration was within ± 4%.CONCLUSIONS:The obtained dynamic upwarp state correction coefficient can be used for dynamic calibration of cyclosporine quality control concentration,which contribute to correct determination of blood concentration of cyclosporine.
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