HPLC法测定二维亚铁颗粒(Ⅱ)中维生素C、维生素B6含量

摘要

目的:建立HPLC测定二维亚铁颗粒(Ⅱ)中维生素C和维生素B6含量的方法.方法:采用Insteril ODS-3色谱柱(250 mm×4.6 mm,5 μm),流动相:0.03 mol·L-1磷酸氢二铵溶液(加入0.01%庚烷磺酸钠,用磷酸调节pH至3.0) -甲醇,梯度洗脱,检测波长为280 nm,流速:0.7～1.0 ml·min-1,柱温30℃.结果:维生素C与维生素B6分别在37～594 μg·ml-1(r=0.999 9)与 3～51 μg·ml-1(r=1.000 0)范围内线性关系良好,平均回收率分别为99.6%与99.2%,RSD分别为0.48%与0.54%(n=9).结论:本法简便、结果准确、可靠,可用于二维亚铁颗粒(Ⅱ)中维生素C、维生素B6的含量测定.%Objective: To establish an HPLC method for the determination of vitamin C and vitamin B6 in ferrous fumarate granules. Method: The procedure was performed on an Insteril ODS-3 column( 250 mm ×4. 6 mm,5 μm )at 30℃ with the detection wavelength at 280 nm. The mobile phase was composed of 0.03 mol·L-1 ammonium phosphate solution ( 0.01% sodium heptanesulfonate was added and the pH was adjusted to 3. 0 by phosphoric acid ) and methanol with gradient elution, and the flow rate was 0. 7-1.0 ml · min-1 . Result: Vitamin C and vitamin B6 were in good linearity within the range of 37-594 μg·ml-1 ( r = 0. 999 9 ) and 3-51 μg· ml-1 ,( r = 1.000 0), respectively. The mean recovery was 99. 6% and 99. 2% with RSD of 0.48% and 0. 54% ( n =9 ) accordingly. Conclusion: The method is simple, accurate, reliable and appropriate in the determination of vitamin C and vitamin B6 in ferrous fumarate granules.