波长变换HPLC法同时测定急支糖浆中6种活性成分

摘要

Objective:To establish an HPLC method for the simultaneous determination of six active constituents ( ferulic acid, chlorogenic acid,ephedrine hydrochloride,protocatechuic acid,protocatechualdehyde and naringin) in Jizhi syrups. Methods:A Zorbax XDB-C18(4.6 mm×250 mm,5 μm)column was used. The mobile phase consisted of acetonitrile (A)-0.9% acetic acid (B) with gradient elution at the flow rate of 1. 0 ml·min-1 . The detection wavelength was changed as follows:257 nm for 0-22. 0 min, 326 nm for 22. 0-30. 0 min, 320 nm for 30. 0-52. 0 min, 210 nm for 52. 0-58. 0 min and 283 nm for 58. 0-60. 0 min. The column temperature was 30 ℃ and the injection volume was 10 μl. Results:The separation of the six active constituents was good. The linear range ( r>0. 9990) was 2. 817-112. 670, 2. 342-93. 670, 0. 710-28. 415, 0. 776-31. 035, 0. 694-27. 755 and 1. 279-51. 175 ng for ferulic acid, chlorogenic acid, ephedrine hydrochloride, protocatechuic acid, protocatechualdehyde and naringin, respectively. The average recover-ies varied from 99.3% to 99.8%(RSD varied from 0.18% to 0.28%). Conclusion: The method is rapid with high sensitivity, promising accuracy and good specificity, which can provide scientific basis for the quality control of Jizhi syrups.%目的:建立同时测定急支糖浆中6种成分(阿魏酸、绿原酸、盐酸麻黄碱、原儿茶酸、原儿茶醛和柚皮苷)的HPLC分析方法.方法:采用Zorbax XDB-C18色谱柱(250 mm×4.6 mm,5μm),以乙腈(A)-0.9%冰醋酸水溶液(B)为流动相,梯度洗脱,流速为1.0 ml·min-1;分段变波长测定:0~22.0 min为257 nm,22.0~30.0 min为326 nm,30.0~52.0 min为320 nm,52.0~58.0 min为210 nm,58.0~60.0 min为283 nm;柱温:30℃,进样量:10μl.结果:阿魏酸、绿原酸、盐酸麻黄碱、原儿茶酸、原儿茶醛和柚皮苷分离度良好,进样量分别在2.817~112.670、2.342~93.670、0.710~28.415、0.776~31.035、0.694~27.755、1.279~51.175 ng线性关系良好,r均>0.9990,平均加样回收率为99.3%~99.8%(RSD为0.18%~0.28%).结论:本法快速、灵敏度高、准确度高、专属性好,为急支糖浆的质量控制提供依据.