高效液相色谱法测定通络祛痛膏中胡椒碱含量

摘要

Objective To establish a method for the determination of Pipeline in Tungluo Qutong Gao by HPLC. Methods The chro-matographic column was Kromasil C18 column(250 mm × 4. 6 mm, 5 m) and the mobile phase was acetonilrile - water (40 : 60) at the flow rate of 1.0mL/min. The UV detection wavelength was at 343 nm. Results The liner range of piperine was 0. 05 ~5 μg( r= 1. 0000); the average recovery rate was 99. 38% (RSD = 1. 04% , n = 9). Conclusion The method is simple and accuate, which can be used for the determination of piperine and for the quality control of Tongluo Qutong Gao.%目的 采用高效液相色谱法测定通络祛痛膏中胡椒碱的含量.方法 采用Kromasil C18色谱柱(250mm×4.6mm,5μm),流动相为乙腈-水(40∶60),流速为1.0mL/min,检测波长为343 nm.结果 胡椒碱质量浓度在0.05 ～5μg(r=1.000 0)范围内与峰面积呈良好线性关系,平均回收率为98.38％,RSD=1.04％(n=9).结论 所用方法简便、准确,可用于测定通络祛痛膏中胡椒碱的含量.