卷柏中穗花杉双黄酮化学对照品的制备方法

摘要

Objective To investigate the preparation method and analysis technique of chemical reference amentoflavone from Selaginella tamariscina(Beauv. ) Spring. Methods The chemical reference amentoflavone was isolated and purified by solvent extract and nhro-matography, which structure was determined by UV, IR, MS,1H - NMR and 13C - NMR. The purity was analyzed by TLC, HPLC. Results The TLC showes the amentoflavone has no impurity spot. The HPLC indicates that it's purity reaches 98.5% above. Conclusion The mothod of isolation and purification for amentoflavone reported in this paper is simple and economical.%目的 研究从卷柏中分离穗花杉双黄酮化学对照品的制备方法与分析技术.方法 采用溶剂和色谱方法从卷柏中分离、纯化、制备穗花杉双黄酮对照品,以紫外、红外光谱、质谱、氢谱和碳谱鉴定结构,以薄层色谱法、高效液相色谱法进行纯度检查.结果 制得的穗花杉双黄酮经薄层色谱法检查无杂质斑点,经高效液相色谱法检测纯度达98.5％以上.结论 所建立的分离、纯化方法简便,成本低.