高效液相色谱法测定妇炎消片中大黄酚和大黄素含量

摘要

Objective To establish the quality standard of Fuyanxiao Tablets. Methods Using HPLC method to determine chrysophanol and emodin content in Fuyanxiao Tablets. C18 column(250 mm x4. 6 mm,5 μm) was adopted; methanol - 0. 1% phosphoric acid (85 : 15) as the mobile phase detection wavelength was 254 nm. Results Linear range of emodin was 0.038 1-0.476 0 μg/mL,and the average recovery rate was 98. 89% , RSD was 0. 78% ( n = 6). Chrysophanol was linear range of 0. 082 4 - 1. 030 0 μg/mL, and the average recovery rate was 99. 01% , RSD was 1. 09% (n = 6). Conclusion The established method is simple,feasible and reproeducible. This study provides a method for the quality control of fuyanxiao Tablets.%目的 初步建立妇炎消片的质量标准.方法 采用高效液相色谱法测定妇炎消片中的大黄酚和大黄素的含量.色谱柱为C18柱(250mm×4.6mm,5μm),以甲醇-0.1%磷酸溶液(85:15)为流动相,流速为1.0mL/min,检测波长为254 nm.结果 大黄素和大黄酚进样量线性范围分别为0.038 1～0.476 0 μg和0.082 4～1.030 0 μg,平均回收率为98.89%和99.01%,RSD分另4为0.78%和1.09%(n=6).结论 该方法简便易行、重现性好,可有效控制该制剂的质量.