Objective To investigate the protective role and mechanism of Shenfu Injection(SF) in ischemia - reperfusion injury of transplanted liver in rats. Methods Male SD rats were divided into three groups: SF group, control group and ischemia - reperfusion injury (I - RI) group. The SF group was infused SF 2 mL/100 g by lumbar vein before getting donor liver. The control group was infused with equivalent 0. 9% sodium chloride injection without any treatment for I - RI group. The level of TNF - a, ALT and AST were detected at 0,60,180 min after liver transplantation. The histopathological damage degree of grafted liver was observed by light microscope. Results The degree of histopathological damage at 180 min after reperfusion in the SF group was lower than that in the control and the I - RI group. The levels of TNF-α, ALT and AST at 60,180 min after reperfusion were significantly decreased in the SF group than the control and I - RI groups (P＜0. 05). Conclusion SF has significant protective effect on transplanted liver in rats via reducing the expression of TNF - α%目的 研究参附注射液对大鼠移植肝脏缺血-再灌注损伤的保护作用及其机制.方法 将雄性SD大鼠分为参附组、对照组和肝脏缺血-再灌注损伤模型组.参附组在获取供肝前经腰静脉灌注参附注射液(2mL/100 g),对照组注入等量0.9%氯化钠注射液,模型组不予处理.光镜观察术后180 min肝脏病理学变化.分别于肝移植完成后0,60,180 min测定大鼠血清丙氨酸氨基转移酶、天门冬酸氨基转移酶和肿瘤坏死因子-α水平.结果 再灌注180 min后,参附组病理损害程度较对照组和模型组轻;再灌注60 min和180 min后,参附组丙氨酸氨基转移酶、天门冬酸氨基转移酶和肿瘤坏死因子-α因子水平明显低于对照组和模型组(P<0.05).结论 参附注射液通过降低炎性因子肿瘤坏死因子-α表达水平,对大鼠移植肝脏缺血-再灌注损伤有明显保护作用.
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