香附、苍术挥发油中α-香附酮和苍术素含量的同时测定

摘要

目的 建立同时测定香附、苍术挥发油中α-香附酮和苍术素含量的高效液相色谱法.方法 以Diamonsil C18柱(150 mm ×4.6 mm,5 μm)为色谱柱,以甲醇-水(74∶26)为流动相,α-香附酮和苍术素的检测波长分别为252 nm和340 nm,流速为1.0 mL/min,柱温为25℃.结果 质量浓度线性范围α-香附酮为0.25 ～ 15.96 μg/mL(r=0.999 9),苍术素为1.55 ～99.00 μg/mL(r =0.999 9).结论 该方法准确、简便,可用于香附、苍术混合挥发油中α-香附酮和苍术素含量的同时测定.%Objective To establish a HPLC method for the simultaneous determination of α-cyperone and atractylodin contents in the volatile oil extracted from Rhizoma Cyperi and Rhizoma Atractylodis.Methods The Diamonsil C18 column(150 mm ×4.6 mm,5 μm) was used as the analytical column.The mobile phase was methanol-water(74:26).The detection wavelength of α-cyperone and atractylodin was at 252 nm and 340 nm,respectively.The flow rate was 1.0 mL/min and the column temperature was at 25 ℃.Results The linear calibration curves of α-cyperone and atractylodin were obtained in the concentration range of 0.25-15.96 μg/mL (r =0.999 9) and 1.55-99.00 μg/mL(r =0.999 9),respectively.Conclusion The established HPLC method is simple,accurate,and can be used to simultaneously determine the content of α-cyperone and atractylodin in the mixed volatile oil extracted from Rhizoma Cyperi and Rhizoma Atractylodis.