高效液相色谱法测定脑脉康颗粒中黄芪甲苷含量

摘要

Objective To establish a method for quality control of Naomaikang Granules.Methods The chromatography column was Agi-lent C18(250 mm ×4.6 mm,5 μm).The mobile phase consisted of Acetonitrile -water(36 ∶64)at a flow rate of 1 mL/min,at 30 ℃. Results Astragaloside IV showed a good linear relationship within the range of 0.0354 mg/mL~0.354 2 mg/mL,equation of linear re-gression was log A=1.602 0 log Ci+3.022 1,R2=0.999 9(n=8),the average recovery was 102.60%,RSD was 2.96%(n=6). Conclusion The methods are simple,exact and reproducible,and can be used for the qualitative and quantitative control of Naomaikang Granules.%目的：建立测定脑脉康颗粒君药黄芪中黄芪甲苷含量的高效液相色谱(HPLC)法。方法色谱柱为Agilent C18柱（250 mm ×4．6 mm，5μm），柱温为30℃，流动相为乙腈-水（36∶64），流速为1 mL／min。结果黄芪甲苷进样质量浓度线性范围是0．0354~0．3542 g／L，线性回归方程为log A＝1．6020 log Ci＋3．0221，r2＝0．9999（n＝8）；平均回收率为102．60％，RSD＝2．96％（n＝6）。结论该方法简便、准确、专属性强，可用于测定脑脉康颗粒君药黄芪中黄芪甲苷的含量。