背景与目的：微小RNA(microRNA, miRNA)在细胞分化、细胞周期及凋亡过程中发挥重要作用。miRNA通过扩增、缺失、突变和沉默等机制影响癌症的发生、发展。本研究探讨miR-187*在结直肠癌组织中的表达和临床意义，以及上调miR-187*表达量对结肠癌细胞凋亡的影响。方法：采用实时荧光定量反转录聚合酶链反应(real-time quantitative reverse transcription-PCR，real-time PCR)的方法在40例结直肠癌患者组织标本中检测miR-187*的表达水平，结合病理资料分析其临床意义。HCT116细胞转染miR-187*mimics后，用Annexin-Ⅴ FITC/PI流式细胞术检测miR-187*对凋亡的影响。结果：miR-187*在结直肠癌组织中表达量为0.165(0.106，0.428)，明显低于正常黏膜组织表达量[0.334(0.211，0.712)]，差异有统计学意义(P<0.05)，且在黏液癌及高龄患者中下调更明显(P<0.05)。将miR-187*mimics转染至HCT116中可提高其表达量，通过流式技术检测发现，与对照组早期凋亡率[(23.010±1.279)%]相比较，实验组早期凋亡率[(26.748±1.098)%]升高，差异有统计学意义(P＜0.05)。结论：miR-187*在结直肠癌组织中低表达，并且与组织学类型及年龄有关；miR-187*表达上调可促进HCT116早期凋亡；miR-187*具有潜在抑癌作用。%Background and purpose: MicroRNAs (miRNAs) play an important role in tumor biological behavior. miRNAs are down-regulated or up-regulated in various cancer types, triggering abnormal cell differentiation, proliferation and apoptosis. This study was designed to investigate the expression and clinical signiifcance of miR-187*in colorectal cancer (CRC), and further to investigate its roles in promoting cell apoptosis. Methods:The expressions of miR-187* in 40 CRC cases were examined by real-time quantitative reverse transcription-PCR (qRT-PCR). The relationship between miR-187*expression and clinical features of CRC was analyzed. HCT116 cells were transfected with a miR-187*mimic and the apoptosis of the transfected cells were examined by lfow cytometry (FCM). Results:The expression of miR-187*was down-regulated in CRC tissues 0.165 (0.106, 0.428) compared with those in normal tissues 0.334 (0.211, 0.712) (P<0.05), especially in mucinous carcinoma and older age CRC (P<0.05). Transfection of HCT116 cells with a miR-187*mimic up-regulated the expression of miR-187*and increased cell early apoptosis (P<0.05). Conclusion: The expression level of miR-187* was lower in CRC. miR-187* expression correlates with histological type and age. Transfection of HCT116 cells with a miR-187*mimic accelerates apoptosis of tumor cells, suggesting that miR-187*is a potent tumor suppressor.
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