12 - methyltetradecanoic acid ( 12 - MTA, anteiso - C15:0 ) possesses notable antitumor activity. The spore - producing bacterium X1 isolated from severe saline - alkali soil was taken as research object, and its taxonomic position was determined by 16S rDNA -based phylogenetic analysis and pheno-typic characteristics. The total fatty acid methyl ester derivatives of X1 were prepared through cell lysis, fat saponification, HC1 - catalyzed methyl esterification and extraction, and their compositions were analyzed by GC - MS. The net weight of total fatty acid methyl ester derivatives of X1 was determined by weighing method. The results showed that the strain X1 was a member of genus Bacillus, and the 16S rDNA similarity data showed it should be a novel species within the genus Bacillus. GC - MS analysis determined that fatty acids of X1 mainly contained four kinds of fatty acids which were iso - C14:0,C14:0,iso -C15:0 and anteiso - C15:0 of which anteiso - C15:0(12 - MTA) was 76. 2% of the total fatty acid content of X1 and 4.47% of X1 dry weight.%12-甲基十四烷酸(12 - MTA,anteiso - C15∶0)具有显著的抗肿瘤活性.以重度盐碱土中分离的产芽孢细菌X1为研究对象,通过16S rDNA系统发育分析和表型特征确定其分类地位,通过细胞裂解、脂肪皂化、盐酸催化的甲酯化和萃取等步骤制备X1菌体总脂肪酸甲酯衍生物,GC - MS分析其组成,结合称重法测定X1菌体总脂肪酸甲酯衍生物净重.结果表明:菌株X1为芽孢杆菌属(Bacillus)的成员,其16S rDNA相似性数据显示其为芽孢杆菌属内的一个新种.GC - MS分析确定X1菌体主要含有iso-C14∶0、C14∶0、iso-C15∶0和anteiso - C15∶0 4种脂肪酸,其中anteiso - C15∶0(12 - MTA)比例占到X1菌体总脂肪酸含量的76.2％,含量占X1菌体干重的4.47％.
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