石榴籽油在D-半乳糖诱导的衰老小鼠体内的抗氧化作用

摘要

The antioxidant activity of Punica granatum seed oil (PGSO) on aging model mice induced by D-galactose was studied.Sixty Kunming mice were randomly divided into six groups:healthy group,model group,vitamin E (VE) positive control group,and low-,medium-,and high-dose PGSO groups.After 45 d treatment,body weights of mice in each group were measured.The effects of PGSO on antioxidant system in liver,kidney,brain and serum of mice were analyzed.And the activity of G6PD and the content of NADPH in liver and kidney of mice were examined.The results showed that PGSO antagonized the decrease of body weight of mice compared with model group.In liver,kidney,brain and serum of mice,PGSO reduced the content of MDA and increased the content of GSH and activity of T-AOC,as well as the activities of antioxidant enzymes SOD and GSH-Px.In addition,in liver and kidney of mice,PGSO elevated the activity of G6PD and the content of NADPH.Taken together,the data indicated that PGSO had the capacity of antagonizing oxidative stress in aging model mice induced by D-galactose.%研究了石榴籽油(PGSO)在D-半乳糖诱导的衰老小鼠体内的抗氧化作用.将60只昆明小鼠随机分为6组:健康组、模型组、维生素E(VE)阳性对照组以及PGSO低、中、高剂量组,持续给药45 d后,测定各组小鼠的体重,分析PGSO对小鼠肝、肾、脑与血清中抗氧化系统的作用;检测各组小鼠肝、肾中G6PD的活性和NADPH含量.结果表明:与模型组比较,PGSO可拮抗小鼠体重的减轻,降低肝、肾、脑及血清中MDA含量,增加GSH含量,提高T-AOC活性以及抗氧化酶SOD和GSH-Px的活性;且PGSO各剂量组小鼠肝和肾中G6PD的活性均增强,NADPH含量均增加.研究表明PGSO对D-半乳糖诱导的衰老小鼠体内的氧化应激具有明显的拮抗作用.