Objective To investigate the effect and mechanism of preoperative Emodin combined with Cisplatin on the expression of wild-type p53-induced gene 1 (WIG-1) in human esophageal squamous cell carcinoma. Methods Forty patients with esophageal squamous cell carcinoma admitted to our hospital from March 2017 to October 2018 were selected as subjects, all patients were diagnosed by preoperative pathological examination. Patients were given Emodin combined with Cisplatin for preoperative chemotherapy after diagnosis. After three consecutive courses of treatment, patients were operated on, according to the different tissues taken during the operation, patients were divided into observation group and control group, with 20 cases in each group. In the observation group, the lesion tissue was taken during the operation, while in the control group, the adjacent tissue was taken during the operation (distance from the tumor site (≥3 cm). The expression of WIG-1 in tissues of the two groups and the expression of WIG-1 before and after operation in the observation group were measured by reverse transcription-polymerase chain reaction (RT-PCR). Results The expression level of WIG-1 in the observation group was 0.68±0.11, higher than 0.04±0.01 in the control group, the difference was statistically significant (P<0.05). The expression level of WIG-1 in esophageal squamous cell carcinoma tissues after operation in the observation group was 0.25±0.05, lower than 0.68±0.11 before operation, the difference was statistically significant (P<0.05). Conclusion The expression level of WIG-1 in normal tissues is low. Preoperative Emodin combined with Cisplatin chemotherapy is helpful to reduce the expression level of WIG-1 in esophageal squamous cell carcinoma patients, improve the prognosis of patients, and provide molecular basis for the treatment of esophageal squamous cell carcinoma.%目的 探讨术前大黄素联合顺铂化疗对人食管鳞癌野生型p53 诱导基因1(WIG-1)mRNA的影响及机制.方法 选择2017年3月～2018年10月我院收治的40例食管鳞癌患者作为研究对象,所有患者均经术前病理组织检查确诊.患者确诊后均给予大黄素联合顺铂进行术前化疗,连续治疗3个疗程后对患者行手术治疗,根据术中所取组织部位的不同,分为观察组和对照组,每组各20例.观察组术中取病灶组织,对照组术中取癌旁组织 (距离肿瘤部位≥3 cm).运用反转录-聚合酶链反应(RT-PCR)技术测定两组患者组织中的WIG-1mRNA 表达水平和观察组手术前后WIG-1mRNA的表达水平.结果 观察组患者组织中的WIG-1mRNA 表达水平为0.68±0.11,高于对照组的0.04±0.01,差异有统计学意义(P<0.05);观察组患者手术后食管鳞癌组织中的WIG-1mRNA 表达水平为0.25±0.05,低于手术前的0.68±0.11,差异有统计学意义(P<0.05).结论 WIG-1mRNA在正常组织中表达水平较低,且术前大黄素联合顺铂化疗用于食管鳞癌患者中有助于降低WIG-1mRNA的表达水平,有助于改善患者的预后,能为食管鳞癌的治疗提供分子学依据.
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