蒸馏-萃取耦合法提取川芎挥发油的工艺优化研究

摘要

Objective To further optimize coupled method of distillation-extraction for volatile oil of Ligusticum Chuanxiong Hort.. Methods Distillation and solvent extraction method were coupled to extract volatile oil of Ligusticum Chuanxiong Hort..Recovery rate of ligustilide was selected as an indicator to optimize the separation and purification of volatile oil of Ligusticum Chuanxiong Hort.,which was mixed with extraction agent.The content of ligustilide was deter-mined by gas chromatography.AT-5 quartz capillary at size of 30 mí0.32 mmí0.25 μm was selected as capillary-col-umn chromatography.The basic conditions were flame ionization detector (FID),temperature of injection port being 250℃, temperature of detector being 250℃,and temperature programming. Results The optimized extraction process was paraffin wax solution extracted once by 1 ml ethyl alcohol,and 5 times by 0.5 ml ethyl alcohol,and salted out by 1.5 times of 30% sodium chloride solution before obtaining volatile oil of Ligusticum Chuanxiong Hort.. Conclusion The opti-mized coupled method of distillation-extraction process is stable and can be applied to extract volatile oil of Ligusticum Chuanxiong Hort..%目的：对蒸馏原萃取耦合法进行进一步优化,用于提取川芎挥发油。方法将蒸馏法与溶剂萃取法耦合用于提取川芎挥发油,以藁本内酯回收率为指标优选混有萃取剂的川芎挥发油的分离、纯化过程。用气相色谱法检测藁本内酯含量,采用AT-5石英毛细管色谱柱(30 m×0.32 mm×0.25μm)；FID检测器；进样口温度：250℃,检测器温度：250℃；程序升温。结果确定优化后的提取工艺,即蒸馏-萃取所得川芎挥发油的石蜡溶液经1 ml乙醇萃取1次,0.5 ml乙醇萃取5次,1.5倍量30%NaCl水溶液盐析等得到川芎挥发油。结论优化后的蒸馏原萃取耦合法工艺稳定,可以用于提取川芎挥发油。