碳酸酐酶Ⅲ、表皮生长因子受体在肺腺癌患者中的表达及其临床意义

摘要

目的：探讨碳酸酐酶Ⅲ(CAⅢ)与表皮生长因子受体(EGFR)在肺腺癌患者中的表达及其临床意义。方法选取本院2011年1月~2015年1月收治的54例肺腺癌患者作为肺癌组，选取同期的50例健康体检者作为对照组，采用酶联免疫吸附试验(ELISA)定量测定CAⅢ水平，采用链霉菌抗生物素蛋白-过氧化物酶连结(SP)免疫组织化学染色法测定EGFR表达，比较两组的CAⅢ水平及EGFR表达，并分析两者与肺腺癌临床病理特征的关系。结果肺癌组的CAⅢ水平、EGFR阳性表达率显著高于对照组，差异有统计学意义(P<0.01)。CAⅢ、EGFR表达与肺腺癌TNM临床分期、组织学分化程度和淋巴结转移等临床病理特征密切相关，TNM临床分期越高、组织学分化程度越低及合并淋巴结转移时，患者的CAⅢ、EGFR表达水平越高。不同临床病理特征患者的CAⅢ、EGFR表达程度比较，差异有统计学意义(P<0.05)。结论 CAⅢ与EGFR在肺腺癌患者中的表达程度明显增强，可作为肺腺癌临床病理特征评估指标。%Objective To explore the expression of carbonic anhydraseⅢ (CAⅢ) and epidermal growth factor recep-tor (EGFR) for lung adenocarcinoma patients and its clinical significance. Methods 54 cases with lung adenocarcinoma in our hospital from January 2011 to January 2015 were selected as the lung cancer group.At the same time,50 cases of healthy people were selected as the control group.The quantitative determination of CA Ⅲ level were detected by en-zyme-linked immunosorbent assay (ELISA),the expression of EGFR were detected by streptavidin biotin protein linked per-oxidase streptavidin peroxidase (SP) immunohistochemical staining.CAⅢlevel and EGFR expression between two groups was compared,then the relationship between CA Ⅲ,EGFR expression and clinical pathological features of lung adeno-carcinoma was analyzed. Results The level of CA Ⅲ and the ratt of EGFR positive expression in the lung cancer group was higher than that in the control group,with significant difference (P<0.01).The expression of CAⅢ and EGFR were closely related to the clinical pathological features of TNM stage,histological differentiation degree and lymph node metastasis,TNM clinical stage was higher,histological differentiation degree was lower and lymph node metastasis, the level of CA Ⅲ and the expression of EGFR were higher,there was significant difference between different clinical pathological features (P<0.05). Conclusion The expression of CA Ⅲ and EGFR in lung adenocarcinoma patients are significantly enhanced,,which can be used as the evaluation index of clinical pathological characteristics of lung adeno-carcinoma.