不同市场紫草相关质量的比较研究

摘要

目的：研究不同市场紫草的相关质量。方法收集10个市场紫草，采用Agilent ZORBAX Extend-C18(4.6 mm×250 mm，5μm)色谱柱，以乙腈-水-甲酸(70：30：0.05)为流动相，检测波长275 nm，柱温25℃，1 ml/min，测定不同市场紫草β，β'-二甲基丙烯酰阿卡宁的含量；采用冷浸法，以95%乙醇为溶剂，测定不同市场紫草浸出物的含量。结果10个不同市场紫草β，β'-二甲基丙烯酰阿卡宁和浸出物含量差异明显，且只有两个市场紫草β，β'-二甲基丙烯酰阿卡宁的含量>0.30%。结论当前市售紫草质量不一，而且很多市场紫草质量令人担忧。%Objective To study the related quality of Arnebiae Radix from different market. Methods Arnebiae Radix from 10 markets were collected,Agilent ZORBAX Extend-C18 (4.6 mmí250 mm,5 μm) chromatographic column was used,acetonitrile-water-formic acid (70:30:0.05) was as the mobile phase,the detection wavelength was 275 nm,column temperature was 25℃,1 ml/min,the content of β,β-dimethylacrylalkannin in A rnebiae Radix from different market was determinated;cooling extracting method was used,95% ethanol was as solvent,the extraction content of Arnebiae Radix from different market was determinated. Results The β,β-dimethylacrylalkannin content and extraction content of A rnebiae Radix from 10 different markets had obvious differences,and there were only two markets β,β-dimethylacry-lalkannin content of Arnebiae Radix more than 0.30%. Conclusion The quality of commercially available Arnebiae Radix is different,and the quality of A rnebiae Radix in a lot of markets is worrying.