索拉非尼对胃癌细胞MGC80-3抑制作用实验研究

摘要

目的：探讨索拉非尼对体外培养人胃癌MGC80-3细胞增殖和凋亡的作用以及作用机制。方法采用二苯基四氮唑溴盐比色法(MTT法)检测不同浓度索拉非尼作用人胃癌MGC80-3细胞的抑制作用；AnnexinV/PI双染法观察药物处理后细胞凋亡率变化。结果索拉非尼对人胃癌MGC80-3细胞增殖具有明显抑制作用，且呈剂量和时间依赖性，与对照组比较差异有统计学意义(孕<0.05)。结论索拉非尼能够诱导细胞凋亡，并随浓度增高凋亡率增加。%Objective To explore the inhibitory effective and mechanism on the proliferation and apoptosis of sorafenib in vitro gastric cancer MGC80-3 cells. Methods The inhibitory effective of sorafenib at different concentrations for gastric cancer cell MGC80-3 were analyzed by MTT assay. Apoptosis rate was observed by AnnexinV/PI staining. Re-sults The proliferation inhibition of sorafenib on gastric cancer MGC80-3 cell was time-dose dependent manner, the difference was statistically significant (P<0.05). Conclusion The apoptosis of gastric cancer MGC80-3 cells is different and the apoptotic rate is increased with the increase of sorafenib concentration.