微囊化胰岛素产生细胞的胰岛素释放情况观察

摘要

目的：微囊化后胰岛素产生细胞（IPCs）的分泌能力的观察。方法分离培养小鼠骨髓间充质干细胞（BM-MSCs）并传代纯化，应用大鼠胰腺损伤提取物（RPE）进行诱导分化，诱导后的细胞随机分成微囊化组和未微囊化组；分别在1、2、3、5、10、15、20、25、30天对微囊化组、未微囊组及BMMSCs组进行胰岛素释放的检测。结果1、2、3、5天微囊化组和未微囊化组都有胰岛素释放并呈上升趋势，两组之间没有明显差异；10 d以后未微囊化组胰岛素释放量有下降的趋势，而微囊化组在第20天胰岛素的释放量开始下降，BM-MSCs组没有胰岛素的释放。结论微囊不会影响细胞的存活，并且能够延长细胞的存活时间和增加细胞的分泌能力。%Objective To study the effects of micro-capsule on the secretion capacity of insulin-producing cells(IPCs). Methods Stem cells from originate mouse bone marrow mesenchymal were isolated,induced and purified. Rat pancre-atic extract(RPE)was extracted from pancreases of rats.BMMSCs were induced by rat pancreatic extract. The induced cells were randomly divided into micro-encapsulated group and non-micro-encapsulated group.The experiment of glu-cose stimulation was performed to detect the level of insulin,respectively, at different time points (1, 2, 3, 5, 10, 15, 20, 25, 30 day).Results The level of insulin secretion was increased after 1, 2, 3, 5 days of culture in micro-encapsu-lated IPCs and non-micro-encapsulated IPCs,but there were no significantly differences among the groups. The level of insulin secretion was declined in non-microencapsulated IPCs at 10 day,while there was no decreased in micro-en-capsulated IPCs until 20 days. Conclusion The micro-capsule can promote the effect duration of IPCs,which supports the function of IPCs.