压力性尿失禁大鼠模型阴道前壁组织中结缔组织生长因子和整合素β1的表达

摘要

目的：探讨结缔组织生长因子(connective tissue growth factor，CTGF)及整合素β1(integrinβ1)在压力性尿失禁(stress urinary incontinence，SUI)大鼠模型阴道前壁中的表达。方法将健康雌性SD大鼠24只随机分为3组，A组大鼠作为对照组不做任何处理；B组大鼠仅行阴道扩张；C组大鼠行阴道扩张加双侧卵巢切除。于术后第4周测定最大膀胱容量(MBC)和腹压漏尿点压(ALPP)，检测压力性尿失禁模型是否建立成功。建模成功4周后处死大鼠后取阴道前壁组织，行免疫组化染色方法和实时荧光定量TR-PCR技术检测CTGF和整合素β1的表达。结果尿流动力学检测(MBC、ALPP)：B、C组较A组明显降低，且各组之间比较均有统计学意义(P<0.05)；CTGF mRNA与蛋白质的表达C组低于A组(P<0.05)，A、B两组之间比较差异无统计学意义(P>0.05)；C组整合素β1 mRNA及蛋白质的表达明显低于A、B组(P<0.05)，且A、B两组之间比较差异有统计学意义(P<0.05)。结论在SUI大鼠阴道前壁CTGF和整合素β1的低表达可能与压力性尿失禁等盆底支持结构的退行性病变的发生存在密切关系。%Objective To investigate the expression of the connective tissue growth factor (CTGF) and integrin β1 in vaginal anterior wall of the model rats with stress urinary incontinence. Methods Twenty-four healthy female SD rats were randomly divided into three groups, group A rats as control group without any treatment;group B rats only with vaginal expansion;group C rats with vaginal expansion and bilateral ovaries removed. At postoperative four weeks to determine the maximum bladder capacity (MBC)and abdominal pressure leakage point pressure (ALPP), to establish detection model for stress urinary incontinence. Modeling successfully executed in rats after four weeks after vaginal anterior wall tissue,immunohistochemical staining method and real-time fluorescent quantitative RT-PCR technique to detect the expression of CTGF and integrinβ1. Results The urine flow dynamic detection(MBC,ALPP)B and C group was significantly lower than group A, there were statistically significant differences (P<0.05);CTGF mRNA and protein expression in group C was significantly lower than group A(P<0.05),there were no statistical significance between group A and group B (P>0 . 05);integrin β1 mRNA and protein in group C expression was low er than that in group A and B(P<0.05), and differences between A and B were statistically significant(P<0.05). Conclusion The anterior wall vaginal CTGF in rats in SUI and low expression of integrin β1 may be related to stress urinary incontinence and other pelvic floor support structure there is a close relationship of the occurrence of degenerative diseases of the pelvic floor support degenerative diseases.