人类偏肺病毒感染与小儿喘息性疾病关系探讨

摘要

目的 了解小儿喘息性疾病人类偏肺病毒(hMPV)感染情况,探讨二者之间关系.方法 对124例小儿喘息性疾病患儿鼻咽分泌物分别作7种常见呼吸道病毒[呼吸道合胞病毒(RSV)、流感病毒A、B型、副流感病毒1、2、3型、腺病毒]免疫荧光检测和hMPV逆转录巢式聚合酶链反应(RT-nPCR)特异性基因检测.结果 共检测到各种病毒阳性70例,其中7种常见呼吸道病毒阳性53例(42.7%),分别是RSV 39例,流感病毒6例,腺病毒5例,副流感病毒3例;RT-nPCR检测hMPV阳性17例(13.7%),hMPV合并RSV 感染2例;RSV和hMPV感染患儿在年龄、性别、临床症状上无统计学意义(P>0.05).结论 RSV仍是小儿喘息性疾病中最多见病原体,hMPV可能是其发病的又一重要病原体,二者临床特征相似.%Objective To investigate correlation between human metapneumovirus infection and wheezing Disease in children. Methods Seven common respiratory viruses extracted from nasopharyngeal secretion in 124children with Wheezing diseases were analyzed with immunofluorescent assay. Meanwhile, the hMPV gene was detected with RT-nPCR. Results Seventy positive cases were detected in all patients. Among them, 53(42.7% )were common respiratory viruses (39 Respiratory syncytial viruses RSV, 6 influenza virus, 5 adenovirus and 3 par influenza virus). Meanwhile, hMPV gene were detected in 17 cases (13.7%). 2 patients were infected with both RSV and hMPV. The age, gender and clinical manifestations of the children infected with RSV and with hMPV didnt show significant difference ( P > 0.05 ). Conclusion RSV was still the most commonly virus in children Wheezing Disease. Meanwhile, hMPV was another important pathogen. The clinical characteristics of the two viruses were similar.