Objective To analyze the expressions of peripheral long-chain non-coding RNA-HOXA transcript at the distal tip (HOTTIP) and microRNA-125b (miRNA-125b) in patients with ST-elevation acute myocardial infarction (STEAMI) undergoing percutaneous coronary intervention (PCI).Methods From June 2015 to February 2016,41 patients with PCI for STEAMI(observation group) and 30 cases without STEAMI (control group) in General Hospital of Ningxia Medical University were enrolled;the observation group was divided into 0-6 h group(11 cases),＞ 6-12 h group(13 cases) and ＞ 12-24 h group(17 cases) according to the duration between STEAMI attack and PCI.Expressions of peripheral HOTTIP and miRNA-125b were detected through real-time fluorescence quantitative polymerase chain reaction.Correlations among HOTTIP,miRNA-125b,cardiac troponin T(cTnT) and high sensitive C reactive protein (hs-CRP) were analyzed.Results Expression levels of HOTTIP and miRNA-125b showed no significant differences among 0-6 h group,＞ 12-24 h group and control group(P ＞ 0.05);the levels in ＞ 6-12 h group were significantly higher than those in control group[(1.09 ± 0.06) vs (0.18 ±0.05),(1.348 ±0.247) vs (0.113 ±0.024)] (P ＜0.05).Expression levels of HOTTIP and miRNA-125b in STEAMI group were significantly higher than those in control group before PCI(P ＜ 0.05);the levels were significantly decreased after PCI [(0.29 ± 0.08) vs (0.78 ± 0.09),(0.093 ± 0.047) vs (0.807 ± 0.043)] (P ＜ 0.05) and there were no significant differences between groups (P ＞ 0.05).After PCI,HOTTIP and miRNA-125b were correlated with cTnT(r =-0.32,P =0.03;r =-0.27,P =0.04);hs-CRP showed no correlations with HOTHP and miRNA-125b(r =-0.13,P =0.31;r =-0.10,P =0.42).Conclusion Expressions of HOTTIP and miRNA-125b increase in STEAMI patients and decrease after PCI;HOTTIP and miRNA-125b may be involved in the process of myocardial ischemia-reperfusion injury in STEAMI.%目的 观察急性ST段抬高型心肌梗死(STEAMI)患者行急诊经皮冠状动脉介入(PCI)治疗前后外周血中长链非编码RNA HOXA远端转录本(HOTIIP)及微小RNA-125b(miRNA-125b)的表达变化,探讨 HOTTIP及miRNA-125b在STEAMI中的作用.方法 入选2015年6月至2016年2月于宁夏医科大学总医院心脏中心内科住院的行急诊PCI治疗的STEAMI患者41例(STEAMI组)及排除STEAMI的患者30例(对照组).STEAMI组患者按照STEAMI发生后行PCI的时间分为0～6h亚组(11例)、＞6～12 h亚组(13例)和＞12 ～24 h亚组(17例).采用实时荧光定量聚合酶链反应检测对照组患者及STEAMI组患者PCI前后外周静脉血中HOTTIP及miRNA-125b的表达水平,分析HOTTIP、miRNA-125b二者之间及其与心肌肌钙蛋白T(cTnT)和高敏C反应蛋白(hs-CRP)的相关性.结果 STEAMI发生后0～6h与＞12 ～24 h,STEAMI组患者HOTTIP及miRNA-125b表达水平与对照组比较,差异均无统计学意义(均P＞0.05);SEAMI发生后＞6 ～ 12 h,HOTTIP及miRNA-125b表达水平明显高于对照组[(1.09±0.06)比(0.18±0.05)、(1.348±0.247)比(0.113±0.024)],差异有统计学意义(P＜0.05).PCI前,STEAMI组HOTTIP及miRNA-125b表达水平明显高于对照组(P＜0.05);PCI后,STEAMI组HOTTIP及miRNA-125b表达水平明U显低于PCI前[(0.29±0.08)比(0.78±0.09)、(0.093±0.047)比(0.807±0.043)],差异有统计学意义(P＜0.05),与对照组比较差异无统计学意义(P＞0.05).STEAMI患者PCI后外周血HOTIP、miRNA-125b与cTnT明显相关(r=-0.32,P=0.03;r=-0.27,P=0.04),与hs-CRP无相关性(r=-0.13,P=0.31;r=-0.10,P=0.42).结论 STEAMI患者的HOTTIP及miRNA-125b表达水平明显升高,行PCI治疗后下降,提示其可能参与了STEAMI心肌缺血再灌注损伤的过程.
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