目的研究姜黄素对 PC-3细胞增殖活性的影响.方法以不同浓度(10、20、40、60、80μmol/L)的姜黄素及顺铂作用于体外培养的 PC-3细胞24 h,以中效浓度(40μmol/L)的姜黄素及顺铂作用于体外培养的 PC-3细胞4、12、24、48、72 h,后用 MTT 法检测在不同浓度及时间作用下 PC-3细胞的抑制率,应用流式细胞仪检测 PC-3细胞周期的变化.结果姜黄素对 PC-3细胞的增殖抑制作用与顺铂相似(P >0.05),具有剂量时间依赖性,并随着药物浓度及时间的增加,抑制率逐渐增高,流式细胞仪检测显示：姜黄素和顺铂组都能使 PC-3细胞生长明显阻滞于 G2/M 期,两组比较差异无统计学意义(P >0.05).各实验组与空白对照组比较均有显著性差异(P <0.05).结论姜黄素可以明显抑制 PC-3细胞的增殖,在临床治疗前列腺癌方面有着良好的应用前景.% Objective To study the inhibitory effect of curcumine on prostate cancer cell line PC-3. Methods By means of different concentrations(10,20,40,60,80μmol/L)of curcumin and Platinol role PC-3 cells for 24 h,by means of lente concentration (40μmol/L) of curcumin and Platinol role PC-3 cells for 4,12,24,48,72 h. MTT method was used to measure the proliferation of PC-3 cells;and detect the cell cycle changes by flow cytometry. Results The depressant effect of curcumine on PC-3 is similar to Platinol(P >0.05),with a concentration and time-dependent. As the drug concentration and time increases,the inhibition rate increased gradually.Flow cytometry showed that,curcumine and Platinol make PC-3 cell growth arrest at G2/M phase, there was no significant difference between the two groups(P >0.05). Each experimental group compared with the control group had significant difference(P <0.05). Conclusion Curcumine can significantly inhibit the proliferation of PC-3 cells and has a better value of clinical application and exploitation in the treatment of prostate cance.
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