Objective To explore the differences of virulence and phospholipase activity of Candida albicans in gastric mucosa of patients with gastric ulcer and different stages’gastric cancer. Methods All the bacterial strain were divided into the normal control group, gastric ulcer group, early gastric cancer group, advanced gastric cancer group according to the sources of strains. The different groups’mucosa strains in CHROMagar candida chromogenic medium were fungal isolated and identificated. And the Candida phospholipase was detected by the egg yolk plate method, the phospholipase activity was indicated with Pz values.The Candida albicans suspension was injected into the tail of the mice, and the virulence was evaluated with the mean survival time. Results Compared with the normal control group, the different groups’phospholipase activity significantly increased, the difference was significant (P<0.05). The phospholipase activity in advanced gastric cancer group was the highest, the toxicity was the strongest, and it could make all the mouse death in 5 days. Conclusion With the increase of malignant degree of gastric mucosa, the phospholipase activity of infecting Candida albicans also elevate, the virulence also increase gradually.%目的：探讨胃溃疡及各期胃癌患者胃黏膜组织中白色念珠菌的毒力及其磷脂酶的活性的差异。方法按照菌株来源分组为正常对照组，胃溃疡组，早期胃癌组，进展期胃癌组，各组胃黏膜的菌株在CHROMagar念珠菌显色培养基上进行真菌分离培养与菌种鉴定，采用蛋黄平板法检测白色念珠菌的磷脂酶，以Pz值表示磷脂酶的活性。用白色念珠菌悬液尾静脉注射感染小鼠，以其平均存活时间（d）评价菌株毒力。结果与正常对照组比较，各组磷脂酶活性均有所升高，差异有统计学意义，以进展期胃癌组的磷脂酶活力最高，毒力最强，可在5d内致全部小鼠死亡。结论随着胃黏膜病理变化恶性程度的升高，所感染白色念珠菌磷脂酶的活性升高，毒力也逐渐增强。
展开▼
