脐血铅浓度与新生儿端粒长度的相关性

摘要

目的：研究脐血铅浓度与新生儿端粒长度的相关性。方法收集2010年7月～2013年4月青岛市市立医院产科孕期无急慢性疾病的足月顺产儿脐血78例，原子吸收光谱法测定脐血铅浓度，测得脐血铅浓度在11～89µg/L范围。将78例脐血分成A、B、C三组：A组脐血铅浓度＜30µg/L，共28例，B组脐血铅浓度30～60µg/L，共38例，C组脐血铅浓度＞60µg/L，共12例。采用全血提取基因组法提取脐血DNA，荧光定量PCR法测基因组DNA的端粒长度。各组间比较采用单因素方差分析，两两比较采用q检验，相关性采用Spearman相关性研究。结果A组端粒长度为：1.152±0.716，B组端粒长度为0.614±0.407，C组端粒长度为0.546±0.339，从A组到C组端粒长度逐渐缩短，且差异有统计学意义（F=4.895，P＜0.05）。B组端粒长度低于A组，差异有统计学意义（P＜0.05）,C组端粒长度低于A组端粒长度，差异有统计学意义（P＜0.01）。B、C两组之间的差异没有统计学意义（P＞0.05）。三组端粒长度与脐血铅浓度呈负相关（r=-0.461，P＜0.01）。结论脐血铅可导致新生儿端粒长度的缩短，且随脐血铅浓度的升高，新生儿端粒长度进行性缩短，端粒长度缩短影响细胞分裂，决定细胞寿命。%Objective To study the correlation of umbilical cord blood lead concentrations and neonatal telomere length. Methods We collected 78 cases of umbilical cord blood from term infants whose gestation was without acute or chronic disease from Qingdao Municipal Hospital between July of 2010 to April of 2013.We used atomic absorption spectrometry to determine the concentration of umbilical cord blood lead. The umbilical cord blood lead concentrations measured range was between 11 and 89 µg/L. The 78 cases of umbilical cord blood was divided into three groups:A,B,and C.Umbilical cord blood lead concentrations for group A was <30µg/L(n=28);group B was 30-60 µg/L (n=38);and group C was>60 µg/L (n=12).To extract umbilical cord blood DNA,extraction genome DNA from whole blood was used. Fluorescence quantitative PCR was used to measure the telomere length of genome DNA.Data were assessed by one-way analysis of variance,differences between groups were compared by q-test and the relationship were analyzed with Spearman correlation research. Results The telomere length of group A was 1.152±0.716,the telomere length of group B was 0.614±0.407,and the telomere length of group C was 0.546±0.339.The telomere length shortened gradually from group A to group C,and the difference was statistically significant(F=4.895,P<0.05). Telomere length of group B was shorter than group A, the difference was statistically significant (P < 0.05),the group C was shorter than group A,the difference was statistically significant (P<0.01).There was no statistical significance between group B and C (P>0.05).The relationship of the three group's telomere length and umbilical cord blood lead concentrations correlated negatively(r=-0.461,P < 0.01). Conclusion Umbilical cord blood lead can shorten the neonatal telomere length. With increasing blood lead concentration,the telomere length of the neonate are progressive shorten which influenced cell division and determined cellular age.