熊果酸诱导慢性粒细胞白血病细胞凋亡过程中活性氧的生物学功能探讨

摘要

目的：研究熊果酸对慢性粒细胞白血病细胞株K562的凋亡及其过程中活性氧自由基的作用。方法不同浓度UA处理后用显微镜观察K562细胞的形态学变化；用Annexin V/PI双染色法检测凋亡；用DCFH－DA法检测活性氧自由基的水平。结果20μmol/L浓度组可见细胞变形、坏死，40μmol/L浓度组可见细胞变形、核固缩、核碎裂和胞膜突起等现象。10、20、40μmol/L作用48h后K562细胞凋亡呈剂量依赖关系，凋亡率分别为21.66％、40.55％、70.21％，随着UA浓度的增高细胞凋亡率也增高，差异具有统计学意义（P＜0.01）。熊果酸能显著增高K562细胞内的活性氧水平，且活性氧水平随着熊果酸的浓度增加而升高。平均荧光强度由48.96升至78.23，差异具有统计学意义（P＜0.01）。结论熊果酸可诱导慢性粒细胞白血病凋亡，且活性氧自由基的水平在凋亡诱导过程中起了重要作用。%Objective To study the effect of UA on apoptosis of ROS cell line K562 and its process.Methods Different concentrations of UA treatment after microscope was used to observe the morphological changes of K562 cells; annexin V/PI double staining method for the detection of apoptosis; variations method was used to detect the reactive oxygen species (ROS) level.Results In the 20μmol/L concentration group, the deformation and necrosis of the cells were found, and the deformation of the cells was 40μmol/L. 10, 20, 40μmol/L for 48h after apoptosis of K562 cells was dose dependent, the apoptosis rate was 21.66%, 40.55%, 70.21%, with UA concentration increased cell apoptosis rate increased. The difference is statistically significant (P<0.01). UA can significantly increase the level of reactive oxygen species in K562 cells, and the level of reactive oxygen species increases with the increase of UA concentration. The average fluorescence intensity was 48.96 to 78.23, and the difference was statistically significant (P<0.01).ConclusionUA can induce the apoptosis of chronic myeloid leukemia, and the level of reactive oxygen species plays an important role in the process of apoptosis induction.