目的:探讨班贞1号和川芎嗪(TMP)联合逆转人胃癌细胞系SGC-7901/ADR对5-FU耐药性与bcl-2表达的相关性.方法:采用半定量逆转录聚合酶链反应(RT-PCR)比较SGC-7901/ADR细胞在单纯5-FU和5-FU联合中药班贞1号及TMP处理组bcl-2 mRNA的表达情况.结果:5-FU组与阴性对照组相比,bcl-2 mRNA表达无显著性差异(P>0.05),而5-FU联合中药班贞1号及TMP处理组bcl-2 mRNA表达量明显降低(P<0.05).结论:班贞1号和TMP联合逆转人胃癌细胞对5-FU耐药性可能与抗调亡分子bcl-2相关.为中西药联合治疗胃癌奠定一定的理论基础.%Objective: To explore of the correlativity between Banzhen No.l and Tetra methylpyrazine (TMP) reversing human gastric cancer cell line SGC-7901/ADR of 5-FU Drug resistance and bcl-2 expressions. Methods: Semi-quantitative Reverse Transcriptase Polymerase Chain Reaction (RT-PCR) was used for comparison of bcl-2 mRNA expression for cell line SGC-7901/ADR in group of 5-FU、5-Fu and Banzhen No.l, Tetramethylpyrazine (TMP). Results: The expression of bcl-2 mRNA between the 5-FU group and the control group, there was no significant differences (ρ＞0.05), while 5-Fu and Banzhen No.l group and etramethylpyrazine (TMP) group was decreased significantly (ρ＜0.05). Conclusion: It is possible correlated to Anti-apoptosis Molecularly bcl-2 for Banzhen No.l and TMP reversed human gastric cancer cells to 5-FU drug resistance, for treatment of gastric cancer with combined Traditional Chinese Medicine and Western Medicine to lay a theoretical foundation.
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