Diagnostic kits and therapeutic retroviral treatment based method, viral protease inhibitors for determining the sensitivity or resistance of retroviral isolates to molecular

摘要

Methods for determining, upstream and downstream of the cleavage site of the precursor amino acid sequence is present, the sequence encoding a protease of a retrovirus should be a) studying the sensitivity or resistance of the isolates of HIV retrovirus to molecules with a portion of its amino acid sequence is present, or without a portion of its amino acid sequence, a step of amplifying a fragment of b) DNA, and the end product of the amplification, to be studied through co-transformation of yeast cells and at least one DNA fragment and the vector, the expression vector that allows under the control of an inducible promoter is known, the expression of sequences encoding a protease of a retrovirus, recombined Transformants arising from cells co-transformed and obtain transformants enough by culturing the yeast cells in which the step, the c) co-transformation, to carry out the testing of the sensitivity or resistance a step of recovering the appropriate media on any, and, in the presence of the molecule to be d) test, to analyze qualitatively or quantitatively a step of incubating the transformant, and e) the cells I and a step of estimating a process, the phenotype of resistance or f) sensitivity. [Selection] Figure Figure 1