外源性胰岛素样生长因子-1对脓毒血症大鼠肺保护的作用

摘要

目的 观察外源性胰岛素样生长因子-1对大鼠脓毒血症引起急性肺损伤的肺保护作用.方法 选择Wistar大鼠40只,随机分为4组(每组各10只):对照组,脓毒血症组,IGF-1治疗1组和治疗2组(50 μg/kg和100μg/kg).采用颈静脉注射脂多糖(LPS,15 mg/kg)制作脓毒血症大鼠ALI模型.注射LPS 12h后,测定各组大鼠血清和支气管肺泡灌洗液IL-6和IL-8浓度.结果 与对照组比较,脓毒血症组大鼠血清IL-6浓度[(25.5±3.71)vs(14.6±2.34)ng/mL]和支气管肺泡灌洗液IL-6浓度[(17.9±2.56) vs (9.1±1.19)ng/mL]均高于对照组,也高于IGF-1治疗组(均P＜0.05),同时脓毒血症组在血清IL-8[(2.03±0.15) vs(1.62±0.13 )ng/mL]和支气管肺泡灌洗液的IL-8浓度[(1.35±0.37)vs(0.98±0.11 )ng/mL]均高于对照组和IGF-1治疗组(均P＜0.05).IGF-1治疗2组大鼠血清和支气管肺泡灌洗液IL-6[(17.3±4.59) vs(17.3±4.59),(11.4±2.93)vs(13.6±2.71 )ng/mL]和IL-8[( 1.73±0.11)vs(1.87±0.26),(0.99±0.39)vs( 1.09±0.43) ng/mL]浓度均低于IGF-1治疗1组(P＜0.05).结论 外源性IGF-1对脓毒血症大鼠引起的急性肺损伤具有保护作用,且随着剂量的加大保护作用更明显.%Objective To study protective effects of insulin-like growth factor-1 (IGF-1) on sepsis induced acute lung injury (ALI) in rats. Methods Forty male SD rats were randomly divided to four groups of control group, model group, IGF-1 treating groups (IGF-1 treating group 1 and IGF-1 treating group 2 with IGF-1 of 50 μg/kg and 100 )JLg/kg, respectively ). The animal model of sepsis induced ALI was established by infusing lipopolysaceharide (LPS) 15 mg/kg via jugular vein. At the 12 h after establishment of models, serum and bronchoalveolar lavage fluid (BALF) IL-6 and IL-8 were determined. Results Compared with normal group, the serum and BALF IL-6 [(25.5±3.71) vs (14.6±2.34), (I7.9± 2.56) vs (9.1±1.19) ng/mL] and IL-8[(2.03±0.15) vs (1.62±0.13), (1.35±0.37) vs (0.98±0.11) ng/mL] were increased in model group and IGF-1 treating groups (P < 0.05). Compared with IGF-1 treating group 2, the serum and BALF IL-6 [(17.3±4.59) vs (17.3±4.59), (11.4±2.93) vs (13.6±2.71) ng/mL] and IL-8 [(1.73±0.11) vs (1.87±0.26), (0.99±0.39) vs (1.09±0.43) ng/mL] were lowered in IGF-1 treating group 1(P < 0.05). Conclusion Exogenous IGF-1 has the protection effect to acute lung injury caused by sepsis rats, and with the increase of dose protection, the effect will be more obvious.