高压氧对脑损伤大鼠BDNF表达的影响

摘要

Objective To observe the effect of hyperbaric oxygen (HBO) treatment on the expression of brain-derived neu rotrophic factor (BDNF) in cerebral cortex and hippocampus of adult rats with lateral fluid percussion attack. Methods Thir ty-two male Wistar rats were divided into brain-injured group and control group with 16 rats in each group. Lateral brain injury model was established in the brain-injured group by fluid percussion attack machine and those rats were divided into two subgroups to be exposed in HBO and nonpressurized air respectively with eight rats in each subgroup. Heads were cut and brains were taken after five days. The morphological changes in cerebral cortex and hippocampus of the brain specimens were observed with electron microscope and the expressions of BDNF were detected with immunohistochemistry. Results There was no significant difference in the number of BDNF positive cells among each normal control groups (P > 0.05). But there were more BDNF positive cells in HBO exposed injury group than that in nonpressurized air exposed group (P< 0.01), and BDNF positive cells in nonpressurized air exposed group were more than that in normal control group (P < 0.01). Conclusion The expression of BDNF in the cortex and hippocampus can be up-regulated by brain injury, which may be further up-regulated by HBO.%目的 研究高压氧(HBO)治疗对侧位液压冲击脑损伤大鼠大脑皮层及海马区脑源性神经营养因子(BDNF)表达的影响.方法 应用液压撞击仪制作侧位液压冲击脑损伤大鼠模型.Wistar雄性大鼠32只,随机分成脑损伤组及正常对照组,每组16只.各组再分别分为两组进行HBO暴露及常压空气暴露,每组8只.5 d后断头取脑,行脑组织切片,应用免疫组化结果 检测BDNF在皮层及海马区的表达.结果正常对照组HBO暴露与常压空气暴露BDNF免疫阳性细胞数比较差异无统计学意义(P > 0.05);脑损伤组中HBO暴露后皮层及海马BDNF免疫阳性细胞数多于常压空气暴露(P < 0.01),脑损伤常压空气暴露组多于正常对照常压空气暴露组(P < 0.01).结论 大鼠脑损伤后可以提高损伤皮层及海马区BDNF的表达,HBO治疗可以进一步提高大鼠脑内BNDF的表达.