Toll样受体4、核转录因子-κB p65在小鼠溃疡性结肠炎中的表达及意义

摘要

目的 探索Toll样受体4(TLR4)和核转录因子-κB(NF-κB)在小鼠溃疡性结肠炎(UC)中的表达及意义,从而探讨该信号通路在UC发病机制中可能的作用.方法 将20只雄性Balb/c小鼠随机分为正常组(N组)和模型组(M组),每组各10只.采用葡聚糖硫酸钠诱导UC模型,各组于造模后7 d取病变处结肠组织,进行组织学评分,HE染色观察大体形态、组织学评分和免疫组化染色观察NF-κB和TLR4的表达情况.结果 TLR4和NF-κB在M组小鼠结肠黏膜表达显著高于N组(P<0.05);且TLR4与NF-κB的表达呈正相关,Pearson相关系数r为0.816.结论 TLR4和NF-κB的高表达提示该信号传导通路激活导致下游炎症因子的释放可能是UC发生的机制之一.%Objective To explore the might mechanism that through the significance of TLR4 and NF-κB p65 expression in the ulcerative colitis. Methods 20 Balb/c mice were randomly divided into normal group (group N) and L/C model group (group M), with 10 mice in each group. Using the DSS to induce UC model, the colonic tissue were collected at 7 d in each group, and the expression of TLR4, NF-κB and pathological change were tested with immimohisi.oehemtsi.ry and HE staining methods and hisiopathologica] score. Results The expression of TLR4 and NF κB in M group was higher than that of group N (P < 0.05), and the expression of TL114 was parallel with that of NF-κB. Pearson correlation coefficient r was 0.816. Conclusion The inflammtory response following TLR4/NF-K.B signaling pathway activated plays a role in the mechanism of UC.